Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 96419..97062 Replicon plasmid pYJ3-a
Accession NZ_AP023228
Organism Escherichia coli strain YJ3

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HXS63_RS23980 Protein ID WP_001034044.1
Coordinates 96646..97062 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HXS63_RS23975 Protein ID WP_001261286.1
Coordinates 96419..96649 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS63_RS23955 93050..93805 - 756 WP_000852146.1 replication initiation protein RepE -
HXS63_RS23960 94527..95333 - 807 WP_000016970.1 site-specific integrase -
HXS63_RS23965 95334..95639 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
HXS63_RS23970 95641..95859 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
HXS63_RS23975 96419..96649 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HXS63_RS23980 96646..97062 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HXS63_RS23985 97137..98702 + 1566 WP_001128474.1 AAA family ATPase -
HXS63_RS23990 98687..99577 + 891 Protein_125 DNA helicase UvrD -
HXS63_RS23995 99656..100455 - 800 Protein_126 IS21-like element IS21 family helper ATPase IstB -
HXS63_RS24000 100455..101629 - 1175 Protein_127 IS21 family transposase -
HXS63_RS24005 101717..101848 + 132 Protein_128 DNA helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / erm(B) / sitABCD / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / tet(B) / catA1 iutA / iucD / iucC / iucB / iucA 1..170909 170909
- inside Integron - iutA / iucD / iucC / iucB / iucA 1..170909 170908


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T36985 WP_001034044.1 NZ_AP023228:96646-97062 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T36985 NZ_AP023228:96646-97062 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT36985 WP_001261286.1 NZ_AP023228:96419-96649 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT36985 NZ_AP023228:96419-96649 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References