Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 95334..95859 Replicon plasmid pYJ3-a
Accession NZ_AP023228
Organism Escherichia coli strain YJ3

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HXS63_RS23965 Protein ID WP_001159871.1
Coordinates 95334..95639 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag HXS63_RS23970 Protein ID WP_000813630.1
Coordinates 95641..95859 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS63_RS23950 91296..92462 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HXS63_RS23955 93050..93805 - 756 WP_000852146.1 replication initiation protein RepE -
HXS63_RS23960 94527..95333 - 807 WP_000016970.1 site-specific integrase -
HXS63_RS23965 95334..95639 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HXS63_RS23970 95641..95859 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HXS63_RS23975 96419..96649 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HXS63_RS23980 96646..97062 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
HXS63_RS23985 97137..98702 + 1566 WP_001128474.1 AAA family ATPase -
HXS63_RS23990 98687..99577 + 891 Protein_125 DNA helicase UvrD -
HXS63_RS23995 99656..100455 - 800 Protein_126 IS21-like element IS21 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / erm(B) / sitABCD / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / tet(B) / catA1 iutA / iucD / iucC / iucB / iucA 1..170909 170909
- inside Integron - iutA / iucD / iucC / iucB / iucA 1..170909 170908


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T36984 WP_001159871.1 NZ_AP023228:c95639-95334 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T36984 NZ_AP023228:c95639-95334 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT36984 WP_000813630.1 NZ_AP023228:c95859-95641 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT36984 NZ_AP023228:c95859-95641 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References