Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4117020..4117596 Replicon chromosome
Accession NZ_AP023226
Organism Escherichia coli strain YJ3

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HXS63_RS19690 Protein ID WP_001297643.1
Coordinates 4117309..4117596 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HXS63_RS19685 Protein ID WP_000063156.1
Coordinates 4117020..4117322 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS63_RS19670 4113616..4115766 + 2151 WP_001446922.1 pyruvate/proton symporter BtsT -
HXS63_RS19675 4115816..4116019 + 204 WP_000467859.1 YbdD/YjiX family protein -
HXS63_RS19680 4116030..4116986 + 957 WP_001297640.1 GTPase -
HXS63_RS19685 4117020..4117322 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HXS63_RS19690 4117309..4117596 - 288 WP_001297643.1 BrnT family toxin Toxin
HXS63_RS19695 4117795..4118492 - 698 Protein_3839 IS1 family transposase -
HXS63_RS19700 4118549..4118644 + 96 Protein_3840 dienelactone hydrolase family protein -
HXS63_RS19705 4118790..4120022 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
HXS63_RS19710 4120063..4121343 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T36974 WP_001297643.1 NZ_AP023226:c4117596-4117309 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T36974 NZ_AP023226:c4117596-4117309 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT36974 WP_000063156.1 NZ_AP023226:c4117322-4117020 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT36974 NZ_AP023226:c4117322-4117020 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References