Detailed information of TA system TA036971

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3668418..3669112
• Replicon: chromosome
• Accession: NZ_AP023226
• Organism: Escherichia coli strain YJ3

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: HXS63_RS17600
• Protein ID: WP_001094398.1
• Coordinates: 3668418..3668786 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HXS63_RS17605
• Protein ID: WP_077249034.1
• Coordinates: 3668807..3669112 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS63_RS17580	3663591..3664547	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
HXS63_RS17585	3664544..3666742	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
HXS63_RS17590	3666752..3667708	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
HXS63_RS17595	3667759..3668097	+	339	Protein_3430	LysR family transcriptional regulator	-
HXS63_RS17600	3668418..3668786	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
HXS63_RS17605	3668807..3669112	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HXS63_RS17610	3669216..3669860	-	645	WP_000086755.1	hypothetical protein	-
HXS63_RS17615	3669879..3670100	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HXS63_RS17620	3670169..3670645	-	477	WP_001424026.1	RadC family protein	-
HXS63_RS17625	3670661..3671134	-	474	WP_000855059.1	antirestriction protein	-
HXS63_RS17630	3671476..3672297	-	822	WP_001234565.1	DUF945 domain-containing protein	-
HXS63_RS17635	3672398..3672606	-	209	Protein_3438	DUF905 family protein	-
HXS63_RS17640	3672707..3673162	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3641152..3669860	28708

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T36971 WP_001094398.1 NZ_AP023226:c3668786-3668418 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T36971 NZ_AP023226:c3668786-3668418 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT36971 WP_077249034.1 NZ_AP023226:c3669112-3668807 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT36971 NZ_AP023226:c3669112-3668807 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4