Detailed information of TA system TA036969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2457678..2458316
• Replicon: chromosome
• Accession: NZ_AP023226
• Organism: Escherichia coli strain YJ3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: HXS63_RS11760
• Protein ID: WP_001447010.1
• Coordinates: 2458140..2458316 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HXS63_RS11755
• Protein ID: WP_001270286.1
• Coordinates: 2457678..2458094 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS63_RS11735	2452830..2453771	-	942	WP_001251304.1	ABC transporter permease	-
HXS63_RS11740	2453772..2454785	-	1014	WP_000220395.1	ABC transporter ATP-binding protein	-
HXS63_RS11745	2454803..2455948	-	1146	WP_000047428.1	ABC transporter substrate-binding protein	-
HXS63_RS11750	2456193..2457599	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HXS63_RS11755	2457678..2458094	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HXS63_RS11760	2458140..2458316	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HXS63_RS11765	2458538..2458768	+	231	WP_000494244.1	YncJ family protein	-
HXS63_RS11770	2458860..2460821	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HXS63_RS11775	2460894..2461430	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HXS63_RS11780	2461483..2462697	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T36969 WP_001447010.1 NZ_AP023226:c2458316-2458140 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T36969 NZ_AP023226:c2458316-2458140 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT36969 WP_001270286.1 NZ_AP023226:c2458094-2457678 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT36969 NZ_AP023226:c2458094-2457678 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8