Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 61048..61649 Replicon plasmid pYJ1-NDM5
Accession NZ_AP023225
Organism Escherichia coli strain YJ1

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag HXS59_RS23555 Protein ID WP_001216034.1
Coordinates 61269..61649 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HXS59_RS23550 Protein ID WP_001190712.1
Coordinates 61048..61269 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS59_RS23540 58029..59312 - 1284 WP_001617890.1 restriction endonuclease subunit S -
HXS59_RS23545 59309..60865 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
HXS59_RS23550 61048..61269 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HXS59_RS23555 61269..61649 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HXS59_RS23560 61654..61833 + 180 WP_001513661.1 hypothetical protein -
HXS59_RS23565 61861..62220 + 360 WP_001513660.1 hypothetical protein -
HXS59_RS23570 62144..62557 + 414 Protein_66 DDE-type integrase/transposase/recombinase -
HXS59_RS23575 63353..64330 + 978 WP_000361610.1 RepB family plasmid replication initiator protein -
HXS59_RS23580 64615..65355 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
HXS59_RS23585 65476..65658 - 183 WP_042065278.1 hypothetical protein -
HXS59_RS23590 66001..66308 - 308 Protein_70 type II toxin-antitoxin system ParD family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(B) / sul1 / qacE / aadA2 / dfrA12 / blaNDM-5 / aadA5 / aac(3)-IId / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 - 1..108281 108281
- flank IS/Tn - - 62183..62557 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T36952 WP_001216034.1 NZ_AP023225:61269-61649 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T36952 NZ_AP023225:61269-61649 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT36952 WP_001190712.1 NZ_AP023225:61048-61269 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT36952 NZ_AP023225:61048-61269 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References