Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 39250..39851 Replicon plasmid pM505-b
Accession NZ_AP023222
Organism Escherichia coli strain M505

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag EPG46_RS24685 Protein ID WP_001216045.1
Coordinates 39250..39630 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag EPG46_RS24690 Protein ID WP_001190712.1
Coordinates 39630..39851 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPG46_RS24660 34690..36174 - 1485 WP_000124155.1 hypothetical protein -
EPG46_RS24665 36174..37367 - 1194 WP_000219605.1 terminase -
EPG46_RS24670 37454..37906 - 453 WP_001369802.1 hypothetical protein -
EPG46_RS24675 37995..39038 - 1044 WP_000648823.1 DUF968 domain-containing protein -
EPG46_RS24680 39066..39245 - 180 WP_000113019.1 hypothetical protein -
EPG46_RS24685 39250..39630 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EPG46_RS24690 39630..39851 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EPG46_RS24695 39924..40313 - 390 WP_000506726.1 DNA repair protein -
EPG46_RS24700 40374..41595 - 1222 Protein_42 ISL3-like element ISKox3 family transposase -
EPG46_RS24705 41762..42013 - 252 WP_001377386.1 DNA polymerase III subunit theta -
EPG46_RS24710 42463..42600 + 138 WP_000123562.1 hypothetical protein -
EPG46_RS24715 42675..43037 - 363 WP_001261544.1 hypothetical protein -
EPG46_RS24720 43034..43957 - 924 WP_176486947.1 hypothetical protein -
EPG46_RS24725 43939..44313 - 375 WP_023154376.1 hypothetical protein -
EPG46_RS24730 44320..44613 - 294 WP_001677496.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..98308 98308


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T36921 WP_001216045.1 NZ_AP023222:c39630-39250 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T36921 NZ_AP023222:c39630-39250 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT36921 WP_001190712.1 NZ_AP023222:c39851-39630 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT36921 NZ_AP023222:c39851-39630 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References