Detailed information of TA system TA036916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 88478..89281
• Replicon: plasmid pM505-a
• Accession: NZ_AP023221
• Organism: Escherichia coli strain M505

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: EPG46_RS24245
• Protein ID: WP_000348883.1
• Coordinates: 88478..89008 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: EPG46_RS24250
• Protein ID: WP_001275013.1
• Coordinates: 89012..89281 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPG46_RS24220	83965..84450	-	486	WP_001403958.1	hypothetical protein	-
EPG46_RS24225	84841..87462	+	2622	WP_001586230.1	hypothetical protein	-
EPG46_RS24230	87661..87891	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
EPG46_RS24235	87980..88255	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
EPG46_RS24240	88260..88477	-	218	Protein_96	transposase	-
EPG46_RS24245	88478..89008	-	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
EPG46_RS24250	89012..89281	-	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
EPG46_RS24255	90169..91158	+	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
EPG46_RS24260	91481..92221	-	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
EPG46_RS24265	92342..92524	-	183	WP_042004909.1	hypothetical protein	-
EPG46_RS24270	92591..92752	-	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EPG46_RS24275	92868..93136	-	269	Protein_103	type II toxin-antitoxin system ParD family antitoxin	-
EPG46_RS24280	93432..94172	-	741	WP_029702141.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / blaTEM-1B / blaCTX-M-15 / qnrS1	-	1..130968	130968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T36916 WP_000348883.1 NZ_AP023221:c89008-88478 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T36916 NZ_AP023221:c89008-88478 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT36916 WP_001275013.1 NZ_AP023221:c89281-89012 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT36916 NZ_AP023221:c89281-89012 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7