Detailed information of TA system TA036915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 87661..88255
• Replicon: plasmid pM505-a
• Accession: NZ_AP023221
• Organism: Escherichia coli strain M505

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EPG46_RS24235
• Protein ID: WP_001403920.1
• Coordinates: 87980..88255 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: EPG46_RS24230
• Protein ID: WP_001261274.1
• Coordinates: 87661..87891 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPG46_RS24215	83164..83936	-	773	Protein_91	hypothetical protein	-
EPG46_RS24220	83965..84450	-	486	WP_001403958.1	hypothetical protein	-
EPG46_RS24225	84841..87462	+	2622	WP_001586230.1	hypothetical protein	-
EPG46_RS24230	87661..87891	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EPG46_RS24235	87980..88255	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
EPG46_RS24240	88260..88477	-	218	Protein_96	transposase	-
EPG46_RS24245	88478..89008	-	531	WP_000348883.1	GNAT family N-acetyltransferase	-
EPG46_RS24250	89012..89281	-	270	WP_001275013.1	DUF1778 domain-containing protein	-
EPG46_RS24255	90169..91158	+	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
EPG46_RS24260	91481..92221	-	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
EPG46_RS24265	92342..92524	-	183	WP_042004909.1	hypothetical protein	-
EPG46_RS24270	92591..92752	-	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EPG46_RS24275	92868..93136	-	269	Protein_103	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / blaTEM-1B / blaCTX-M-15 / qnrS1	-	1..130968	130968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T36915 WP_001403920.1 NZ_AP023221:87980-88255 [Escherichia coli]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T36915 NZ_AP023221:87980-88255 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT36915 WP_001261274.1 NZ_AP023221:87661-87891 [Escherichia coli]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT36915 NZ_AP023221:87661-87891 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1