Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4513687..4514263 Replicon chromosome
Accession NZ_AP023219
Organism Escherichia coli strain M505

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EPG46_RS21590 Protein ID WP_001297643.1
Coordinates 4513687..4513974 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EPG46_RS21595 Protein ID WP_000063156.1
Coordinates 4513961..4514263 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPG46_RS21570 4509940..4511220 - 1281 WP_001037380.1 DUF445 domain-containing protein -
EPG46_RS21575 4511261..4512493 - 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
EPG46_RS21580 4512639..4512734 - 96 Protein_4185 dienelactone hydrolase family protein -
EPG46_RS21585 4512791..4513488 + 698 Protein_4186 IS1 family transposase -
EPG46_RS21590 4513687..4513974 + 288 WP_001297643.1 BrnT family toxin Toxin
EPG46_RS21595 4513961..4514263 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EPG46_RS21600 4514297..4515253 - 957 WP_001297640.1 GTPase -
EPG46_RS21605 4515264..4515467 - 204 WP_000467859.1 YbdD/YjiX family protein -
EPG46_RS21610 4515517..4517667 - 2151 WP_001446922.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T36909 WP_001297643.1 NZ_AP023219:4513687-4513974 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T36909 NZ_AP023219:4513687-4513974 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT36909 WP_000063156.1 NZ_AP023219:4513961-4514263 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT36909 NZ_AP023219:4513961-4514263 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References