Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2930057..2930711 Replicon chromosome
Accession NZ_AP023219
Organism Escherichia coli strain M505

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EPG46_RS14010 Protein ID WP_000244777.1
Coordinates 2930057..2930464 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EPG46_RS14015 Protein ID WP_000354046.1
Coordinates 2930445..2930711 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPG46_RS13990 2926014..2927747 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
EPG46_RS13995 2927753..2928463 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EPG46_RS14000 2928488..2929384 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EPG46_RS14005 2929496..2930017 + 522 WP_001055874.1 flavodoxin FldB -
EPG46_RS14010 2930057..2930464 - 408 WP_000244777.1 toxin CptA Toxin
EPG46_RS14015 2930445..2930711 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EPG46_RS14020 2930954..2931934 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EPG46_RS14025 2932130..2932789 - 660 WP_000250274.1 hemolysin III family protein -
EPG46_RS14030 2932953..2933264 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
EPG46_RS14035 2933309..2934742 + 1434 WP_001446971.1 6-phospho-beta-glucosidase BglA -
EPG46_RS14040 2934799..2935542 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T36903 WP_000244777.1 NZ_AP023219:c2930464-2930057 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T36903 NZ_AP023219:c2930464-2930057 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT36903 WP_000354046.1 NZ_AP023219:c2930711-2930445 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT36903 NZ_AP023219:c2930711-2930445 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References