Detailed information of TA system TA036889

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 134940..135775
• Replicon: chromosome
• Accession: NZ_AP023219
• Organism: Escherichia coli strain M505

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: EPG46_RS00645
• Protein ID: WP_000854726.1
• Coordinates: 135398..135775 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L6W4Z7
• Locus tag: EPG46_RS00640
• Protein ID: WP_070749977.1
• Coordinates: 134940..135308 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPG46_RS00610	131815..132633	+	819	WP_070749978.1	DUF945 domain-containing protein	-
EPG46_RS00615	132633..132878	+	246	WP_000680584.1	hypothetical protein	-
EPG46_RS00620	132972..133451	+	480	WP_000706976.1	antirestriction protein	-
EPG46_RS00625	133465..133941	+	477	WP_001186742.1	RadC family protein	-
EPG46_RS00630	134010..134231	+	222	WP_000692353.1	DUF987 domain-containing protein	-
EPG46_RS00635	134246..134890	+	645	Protein_115	antitoxin of toxin-antitoxin stability system	-
EPG46_RS00640	134940..135308	+	369	WP_070749977.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EPG46_RS00645	135398..135775	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
EPG46_RS00650	135940..136689	-	750	Protein_118	IS21-like element ISEc12 family helper ATPase IstB	-
EPG46_RS00655	136704..138247	-	1544	Protein_119	IS21-like element ISEc12 family transposase	-
EPG46_RS00660	138441..138851	+	411	Protein_120	hypothetical protein	-
EPG46_RS00665	138863..139060	+	198	WP_000839267.1	DUF957 domain-containing protein	-
EPG46_RS00670	139145..139993	+	849	WP_001280510.1	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	107233..147966	40733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T36889 WP_000854726.1 NZ_AP023219:135398-135775 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T36889 NZ_AP023219:135398-135775 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13860.63 Da
• Isoelectric Point: 6.3177

>AT36889 WP_070749977.1 NZ_AP023219:134940-135308 [Escherichia coli]
VSDTFHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQVFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT36889 NZ_AP023219:134940-135308 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATTTGGACCAGGTCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGGTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: A0A1L6W4Z7