Detailed information of TA system TA036883

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 83450..84157
• Replicon: plasmid pMTY18781-2
• Accession: NZ_AP023207
• Organism: Escherichia coli strain TUM18781

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HUV20_RS21870
• Protein ID: WP_089586169.1
• Coordinates: 83816..84157 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8S7X7W3
• Locus tag: HUV20_RS21865
• Protein ID: WP_053264533.1
• Coordinates: 83450..83785 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUV20_RS21840	79397..79615	-	219	WP_001064742.1	AlpA family phage regulatory protein	-
HUV20_RS21845	79733..80347	-	615	WP_000772910.1	inovirus Gp2 family protein	-
HUV20_RS21850	80940..81893	+	954	WP_053264531.1	hypothetical protein	-
HUV20_RS21855	82129..82947	+	819	WP_053264532.1	DUF945 domain-containing protein	-
HUV20_RS21860	82977..83450	+	474	WP_001296921.1	DNA repair protein RadC	-
HUV20_RS21865	83450..83785	+	336	WP_053264533.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUV20_RS21870	83816..84157	+	342	WP_089586169.1	TA system toxin CbtA family protein	Toxin
HUV20_RS21875	84272..85105	+	834	WP_053264535.1	DUF4942 domain-containing protein	-
HUV20_RS21880	85175..85570	+	396	WP_000152741.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
HUV20_RS21885	85563..86510	+	948	WP_000342497.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HUV20_RS21890	87112..87762	+	651	WP_001037963.1	HNH endonuclease	-
HUV20_RS21895	87770..88750	-	981	WP_053264536.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fimB / fimE	1..93845	93845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13104.13 Da
• Isoelectric Point: 9.4583

>T36883 WP_089586169.1 NZ_AP023207:83816-84157 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDTINFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCYNTTAR

Nucleotide

• Length: 342 bp

>T36883 NZ_AP023207:83816-84157 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGAATCACTCTGGTCGATACCATCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCTG
CTATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12593.55 Da
• Isoelectric Point: 8.4816

>AT36883 WP_053264533.1 NZ_AP023207:83450-83785 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPRQQR
TVTLYAKGLTCEADTLGSSGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT36883 NZ_AP023207:83450-83785 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCCGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCAGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA