Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4397528..4398025 Replicon chromosome
Accession NZ_AP023197
Organism Escherichia coli strain TUM18780

Toxin (Protein)


Gene name ghoT Uniprot ID A0A163EQM5
Locus tag HUV22_RS20750 Protein ID WP_001672275.1
Coordinates 4397528..4397701 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUV22_RS20755 Protein ID WP_000398619.1
Coordinates 4397729..4398025 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUV22_RS20740 4394198..4395655 + 1458 WP_021557456.1 dipeptide/tripeptide permease DtpC -
HUV22_RS20745 4395892..4397409 + 1518 WP_001295074.1 lysine--tRNA ligase -
HUV22_RS20750 4397528..4397701 - 174 WP_001672275.1 type V toxin-antitoxin system toxin GhoT Toxin
HUV22_RS20755 4397729..4398025 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUV22_RS20760 4398252..4398524 - 273 WP_000405647.1 N-acetyltransferase -
HUV22_RS20765 4398536..4398766 - 231 WP_021557455.1 (4Fe-4S)-binding protein -
HUV22_RS20770 4398947..4400578 + 1632 WP_001216477.1 sensor histidine kinase -
HUV22_RS20775 4400575..4401294 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4370011..4398025 28014


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6569.09 Da        Isoelectric Point: 10.2288

>T36848 WP_001672275.1 NZ_AP023197:c4397701-4397528 [Escherichia coli]
MALFSKILIFYVIGVNISFIIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T36848 NZ_AP023197:c4397701-4397528 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTATCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT36848 WP_000398619.1 NZ_AP023197:c4398025-4397729 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT36848 NZ_AP023197:c4398025-4397729 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTGAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A163EQM5

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References