Detailed information of TA system TA036845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4067069..4067619
• Replicon: chromosome
• Accession: NZ_AP023197
• Organism: Escherichia coli strain TUM18780

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: HUV22_RS19165
• Protein ID: WP_001160975.1
• Coordinates: 4067069..4067383 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D3H431
• Locus tag: HUV22_RS19170
• Protein ID: WP_000125565.1
• Coordinates: 4067386..4067619 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUV22_RS19140	4062744..4064030	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
HUV22_RS19145	4064030..4065019	+	990	WP_021556398.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
HUV22_RS19150	4065016..4065837	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
HUV22_RS19155	4065840..4066217	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
HUV22_RS19160	4066224..4067072	+	849	WP_021556397.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
HUV22_RS19165	4067069..4067383	-	315	WP_001160975.1	CcdB family protein	Toxin
HUV22_RS19170	4067386..4067619	-	234	WP_000125565.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HUV22_RS19175	4067705..4068184	-	480	WP_001487721.1	type 3 dihydrofolate reductase	-
HUV22_RS19180	4068376..4070238	-	1863	WP_040234481.1	glutathione-regulated potassium-efflux system protein KefC	-
HUV22_RS19185	4070231..4070761	-	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
HUV22_RS19190	4070872..4072203	-	1332	WP_001183198.1	MFS transporter	-
HUV22_RS19195	4072261..4072548	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T36845 WP_001160975.1 NZ_AP023197:c4067383-4067069 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T36845 NZ_AP023197:c4067383-4067069 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8599.64 Da
• Isoelectric Point: 6.9791

>AT36845 WP_000125565.1 NZ_AP023197:c4067619-4067386 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Nucleotide

• Length: 234 bp

>AT36845 NZ_AP023197:c4067619-4067386 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGCGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A090NGC7