Detailed information of TA system TA036832

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1833316..1834084
• Replicon: chromosome
• Accession: NZ_AP023197
• Organism: Escherichia coli strain TUM18780

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HUV22_RS08585
• Protein ID: WP_000854814.1
• Coordinates: 1833316..1833690 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HUV22_RS08590
• Protein ID: WP_174402693.1
• Coordinates: 1833779..1834084 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUV22_RS08545	1828712..1829878	+	1167	WP_085668764.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HUV22_RS08550	1829997..1830470	+	474	WP_001105393.1	DNA gyrase inhibitor SbmC	-
HUV22_RS08555	1830668..1831726	+	1059	WP_085668766.1	FUSC family protein	-
HUV22_RS08560	1831898..1832227	+	330	WP_000450409.1	DUF496 family protein	-
HUV22_RS08565	1832328..1832510	-	183	WP_001016348.1	hypothetical protein	-
HUV22_RS08570	1832582..1832710	+	129	Protein_1670	transposase domain-containing protein	-
HUV22_RS08575	1832999..1833112	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HUV22_RS08580	1833125..1833319	-	195	WP_000988600.1	hypothetical protein	-
HUV22_RS08585	1833316..1833690	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HUV22_RS08590	1833779..1834084	-	306	WP_174402693.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUV22_RS08595	1834223..1834444	-	222	WP_000692318.1	DUF987 domain-containing protein	-
HUV22_RS08600	1834513..1834989	-	477	WP_001186725.1	RadC family protein	-
HUV22_RS08605	1835005..1835484	-	480	WP_000860087.1	antirestriction protein	-
HUV22_RS08610	1835566..1836384	-	819	WP_174402631.1	DUF945 domain-containing protein	-
HUV22_RS08615	1836484..1836717	-	234	WP_032173243.1	DUF905 family protein	-
HUV22_RS08620	1836796..1837251	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (2-96)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T36832 WP_000854814.1 NZ_AP023197:c1833690-1833316 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T36832 NZ_AP023197:c1833690-1833316 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11294.04 Da
• Isoelectric Point: 7.2884

>AT36832 WP_174402693.1 NZ_AP023197:c1834084-1833779 [Escherichia coli]
MPCTVTPFFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKA
DTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 306 bp

>AT36832 NZ_AP023197:c1834084-1833779 [Escherichia coli]
CTGCCCTGCACCGTGACGCCCTTTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGC
CGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAAC
TCATGCTCACCAGCGGTGAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCC
GATACCCTCAGCAGTTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50