Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 287779..288410 Replicon chromosome
Accession NZ_AP023197
Organism Escherichia coli strain TUM18780

Toxin (Protein)


Gene name hicA Uniprot ID A0A1M1UJK7
Locus tag HUV22_RS01235 Protein ID WP_001259382.1
Coordinates 287779..288054 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HUV22_RS01240 Protein ID WP_000593555.1
Coordinates 288051..288410 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUV22_RS01220 282783..283850 + 1068 WP_000361517.1 HlyD family secretion protein -
HUV22_RS01225 283847..286582 + 2736 WP_000149130.1 ribosome-associated ATPase/putative transporter RbbA -
HUV22_RS01230 286582..287706 + 1125 WP_021557290.1 ABC transporter permease -
HUV22_RS01235 287779..288054 + 276 WP_001259382.1 type II toxin-antitoxin system HicA family toxin Toxin
HUV22_RS01240 288051..288410 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUV22_RS01245 288460..289320 - 861 WP_021557289.1 class II aldolase -
HUV22_RS01250 289352..289621 - 270 WP_000933060.1 HPr family phosphocarrier protein -
HUV22_RS01255 289611..291119 - 1509 WP_021557288.1 xylulose kinase -
HUV22_RS01260 291112..292470 - 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
HUV22_RS01265 292547..292828 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
HUV22_RS01270 292825..293298 - 474 WP_001161650.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.91 Da        Isoelectric Point: 10.7076

>T36827 WP_001259382.1 NZ_AP023197:287779-288054 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T36827 NZ_AP023197:287779-288054 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT36827 WP_000593555.1 NZ_AP023197:288051-288410 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT36827 NZ_AP023197:288051-288410 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M1UJK7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References