Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3433180..3433801 Replicon chromosome
Accession NZ_AP023184
Organism Buttiauxella agrestis strain DSM 9389

Toxin (Protein)


Gene name Hha Uniprot ID A0A085GI74
Locus tag BADSM9389_RS15910 Protein ID WP_034493744.1
Coordinates 3433583..3433801 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A085GI75
Locus tag BADSM9389_RS15905 Protein ID WP_034493745.1
Coordinates 3433180..3433554 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BADSM9389_RS15895 3428255..3429454 + 1200 WP_034493747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BADSM9389_RS15900 3429477..3432626 + 3150 WP_172895369.1 multidrug efflux RND transporter permease subunit AcrB -
BADSM9389_RS15905 3433180..3433554 + 375 WP_034493745.1 Hha toxicity modulator TomB Antitoxin
BADSM9389_RS15910 3433583..3433801 + 219 WP_034493744.1 hemolysin expression modulator Hha Toxin
BADSM9389_RS15915 3434131..3434685 + 555 WP_115630472.1 maltose O-acetyltransferase -
BADSM9389_RS15920 3434788..3435246 + 459 WP_172895370.1 hypothetical protein -
BADSM9389_RS15925 3435272..3435412 - 141 WP_072011136.1 type B 50S ribosomal protein L36 -
BADSM9389_RS15930 3435709..3436746 + 1038 WP_172895371.1 isopenicillin N synthase family oxygenase -
BADSM9389_RS15935 3436765..3437574 + 810 WP_034493740.1 MetQ/NlpA family ABC transporter substrate-binding protein -
BADSM9389_RS15940 3437571..3438362 + 792 WP_172895372.1 ATP-binding cassette domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8582.96 Da        Isoelectric Point: 8.9108

>T36781 WP_034493744.1 NZ_AP023184:3433583-3433801 [Buttiauxella agrestis]
MTTKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWSFIR

Download         Length: 219 bp

>T36781 NZ_AP023184:3433583-3433801 [Buttiauxella agrestis]
ATGACTACAAAACCATTAACAAAGACCGATTATTTGATGCGCCTGCGACGCTGCCAAACAATTGATACATTAGAACGAGT
GATTGAAAAGAATAAATACGAATTATCAGATAATGAATTGGCAGTATTTTATTCAGCCGCAGACCACCGTCTGGCAGAGC
TGACCATGAATAAGCTGTACGATAAAATACCGCCTTCTGTATGGTCATTTATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14530.31 Da        Isoelectric Point: 4.7295

>AT36781 WP_034493745.1 NZ_AP023184:3433180-3433554 [Buttiauxella agrestis]
MDEYSPKRHDIAQLKFLCENMYHDCLTNLGESNHGWVNDPTSAINLQLNELIEHIAAFALNYKIKYDDESKLIEQIDEYL
DDTFMLFSNYGINAQDLQKWRKSGNRLFRCFVTESRANPVSLSF

Download         Length: 375 bp

>AT36781 NZ_AP023184:3433180-3433554 [Buttiauxella agrestis]
ATGGACGAATACTCACCAAAACGGCATGATATCGCGCAGCTTAAGTTTCTCTGCGAAAACATGTATCATGACTGCCTTAC
CAACCTTGGCGAGAGTAATCATGGTTGGGTTAATGATCCTACTTCGGCCATTAACTTGCAGTTGAATGAATTGATTGAGC
ACATTGCGGCATTCGCACTTAATTACAAAATTAAGTACGATGATGAGAGTAAATTGATTGAACAAATTGACGAATATTTG
GACGATACATTTATGCTGTTCAGTAACTACGGTATCAATGCCCAGGATCTTCAGAAATGGAGAAAGTCTGGAAATCGATT
ATTCCGATGCTTCGTTACTGAGAGCCGCGCAAACCCTGTAAGCCTCTCCTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A085GI74

Antitoxin

Source ID Structure
AlphaFold DB A0A085GI75

References