Detailed information of TA system TA036767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 279304..279923
• Replicon: chromosome
• Accession: NZ_AP023184
• Organism: Buttiauxella agrestis strain DSM 9389

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BADSM9389_RS01250
• Protein ID: WP_172893006.1
• Coordinates: 279304..279687 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BADSM9389_RS01255
• Protein ID: WP_172893007.1
• Coordinates: 279681..279923 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BADSM9389_RS01220	274619..275524	+	906	WP_034499262.1	formate dehydrogenase subunit beta	-
BADSM9389_RS01225	275521..276156	+	636	WP_034499260.1	formate dehydrogenase cytochrome b556 subunit	-
BADSM9389_RS01230	276153..277082	+	930	WP_172893004.1	formate dehydrogenase accessory protein FdhE	-
BADSM9389_RS01235	277143..278051	+	909	WP_172893005.1	alpha/beta hydrolase	-
BADSM9389_RS01240	278131..278691	-	561	WP_172896068.1	RNA polymerase sigma factor	-
BADSM9389_RS01245	278697..279152	-	456	WP_172896069.1	hypothetical protein	-
BADSM9389_RS01250	279304..279687	-	384	WP_172893006.1	type II toxin-antitoxin system VapC family toxin	Toxin
BADSM9389_RS01255	279681..279923	-	243	WP_172893007.1	ribbon-helix-helix domain-containing protein	Antitoxin
BADSM9389_RS01260	280143..280364	-	222	WP_034499252.1	YgdI/YgdR family lipoprotein	-
BADSM9389_RS01265	280488..280979	+	492	WP_172893008.1	hypothetical protein	-
BADSM9389_RS01270	281137..281667	-	531	WP_172893009.1	hypothetical protein	-
BADSM9389_RS01275	281797..281976	+	180	WP_172893010.1	toxin-antitoxin system HicB family antitoxin	-
BADSM9389_RS01280	282005..282292	+	288	WP_172893011.1	hypothetical protein	-
BADSM9389_RS01285	282465..284294	-	1830	WP_172893012.1	hypothetical protein	-
BADSM9389_RS01290	284278..284760	-	483	WP_172893013.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-117)

Antitoxin

• Ribbon-helix-helix protein, CopG (16-49)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14180.45 Da
• Isoelectric Point: 8.5121

>T36767 WP_172893006.1 NZ_AP023184:c279687-279304 [Buttiauxella agrestis]
MVKMMQSAVFDTNILIDYLKGIPQARATIESYGHSPSISIVTWMEVMVGAKHHDQEQQTRHFLSGFEILPVNQAVAEHSV
ALRQQYGMKLPDAIILASTQISARKLITRNSKDFKDLPGVILPYSLG

Nucleotide

• Length: 384 bp

>T36767 NZ_AP023184:c279687-279304 [Buttiauxella agrestis]
ATGGTGAAGATGATGCAATCTGCTGTTTTTGATACCAATATTTTAATCGATTATCTGAAAGGGATCCCGCAAGCGCGTGC
AACCATTGAAAGCTACGGTCATTCCCCATCAATTAGCATTGTGACCTGGATGGAAGTCATGGTCGGGGCAAAACACCACG
ATCAGGAACAGCAGACCCGGCATTTCCTGAGTGGATTTGAAATTTTGCCGGTCAATCAGGCGGTGGCAGAACACTCTGTA
GCGCTACGTCAGCAATATGGAATGAAACTACCTGATGCCATTATTTTGGCCAGTACGCAGATCAGTGCCAGGAAACTCAT
CACTCGAAATTCAAAAGACTTTAAGGATCTGCCAGGCGTAATTTTGCCTTATTCCCTGGGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9374.65 Da
• Isoelectric Point: 4.3648

>AT36767 WP_172893007.1 NZ_AP023184:c279923-279681 [Buttiauxella agrestis]
MATMFGEMMGRILLDLSDEAISRLDSLKQKRNLPRAELLREAVEEYLERQAQTSIKEAFGLWGEDAPDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT36767 NZ_AP023184:c279923-279681 [Buttiauxella agrestis]
ATGGCAACGATGTTTGGAGAGATGATGGGACGAATTCTTCTGGATCTGTCTGATGAGGCAATCAGTCGCCTTGATAGCCT
TAAACAGAAACGCAATTTACCGCGAGCAGAGCTTTTACGAGAAGCGGTCGAGGAGTATCTGGAAAGGCAGGCTCAAACCA
GTATCAAAGAAGCATTTGGTCTTTGGGGCGAAGATGCACCTGATGGTTTGGAATATGAACGCAAACTTCGCGAGGAATGG
TGA