Detailed information of TA system TA036756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 156684..157219
• Replicon: plasmid RQCS_2
• Accession: NZ_AP023173
• Organism: Rhodococcus qingshengii strain CS98

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7I8BDH8
• Locus tag: RQCS_RS29670
• Protein ID: WP_042445814.1
• Coordinates: 156684..156983 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: RQCS_RS29675
• Protein ID: WP_064688354.1
• Coordinates: 156983..157219 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RQCS_RS29655	152808..153878	+	1071	WP_064688351.1	DUF1016 family protein	-
RQCS_RS29660	154008..154805	-	798	WP_064688352.1	hypothetical protein	-
RQCS_RS29665	154805..156655	-	1851	WP_064688353.1	hypothetical protein	-
RQCS_RS29670	156684..156983	-	300	WP_042445814.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RQCS_RS29675	156983..157219	-	237	WP_064688354.1	hypothetical protein	Antitoxin
RQCS_RS29680	157595..158269	-	675	WP_064688355.1	TetR/AcrR family transcriptional regulator	-
RQCS_RS29685	158325..159299	-	975	WP_064688356.1	DUF2637 domain-containing protein	-
RQCS_RS29690	159922..160236	-	315	WP_064444977.1	hypothetical protein	-
RQCS_RS29695	160233..161015	-	783	WP_029256883.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..485693	485693

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10441.94 Da
• Isoelectric Point: 4.3831

>T36756 WP_042445814.1 NZ_AP023173:c156983-156684 [Rhodococcus qingshengii]
MRPIHEADLDTVRPVLVLTREVVRPHLGTVTVAPITDEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNVIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T36756 NZ_AP023173:c156983-156684 [Rhodococcus qingshengii]
ATGCGACCAATTCACGAAGCCGACCTCGACACAGTCCGCCCGGTCCTCGTACTCACCCGAGAGGTGGTCCGTCCACACCT
CGGGACCGTCACGGTAGCGCCCATCACCGACGAGATCCGAGGACTGTCCACTGAGGTGTCCGTCAGTGCCGCGAACGGAC
TATCCGCCGACGCGGTGGTCAGTTGCGATCACATCACCACCATTCCCGTCAGTGCACTCGGCAACGTCATCGGTTATCTT
CTGCCAGCACAAGAAGAATCACTGTCCATAGCCGTGCGCGCCGCATTCGATCTCGAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8642.63 Da
• Isoelectric Point: 6.4809

>AT36756 WP_064688354.1 NZ_AP023173:c157219-156983 [Rhodococcus qingshengii]
MSTQIAVRLPDEIVAFLDDEIREHRATSRASVVLRALERERRRQIAARDAEILANATSEHDLSSLAHHAANPQSTGLS

Nucleotide

• Length: 237 bp

>AT36756 NZ_AP023173:c157219-156983 [Rhodococcus qingshengii]
ATGAGCACGCAGATCGCGGTACGACTACCGGACGAGATCGTGGCATTTCTCGACGACGAAATCAGGGAACATCGCGCCAC
CTCGCGAGCCTCGGTAGTGTTGCGCGCTCTCGAGCGCGAACGTCGCCGTCAGATTGCCGCACGTGACGCGGAGATTCTCG
CGAACGCCACATCCGAACATGACCTGAGTTCTCTCGCTCACCACGCCGCCAATCCGCAGTCAACCGGCTTGAGCTGA

Structures

Toxin

• AlphaFold DB: A0A7I8BDH8