Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3841069..3841688 Replicon chromosome
Accession NZ_AP023148
Organism Klebsiella pneumoniae strain SMKP03

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag SMKP03_RS18630 Protein ID WP_002892050.1
Coordinates 3841470..3841688 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag SMKP03_RS18625 Protein ID WP_002892066.1
Coordinates 3841069..3841443 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SMKP03_RS18615 3836221..3837414 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
SMKP03_RS18620 3837437..3840583 + 3147 WP_172879345.1 multidrug efflux RND transporter permease subunit AcrB -
SMKP03_RS18625 3841069..3841443 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
SMKP03_RS18630 3841470..3841688 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
SMKP03_RS18635 3841847..3842413 + 567 WP_002892030.1 maltose O-acetyltransferase -
SMKP03_RS18640 3842385..3842525 - 141 WP_004147370.1 hypothetical protein -
SMKP03_RS18645 3842546..3843016 + 471 WP_002892026.1 YlaC family protein -
SMKP03_RS18650 3842991..3844442 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
SMKP03_RS18655 3844543..3845241 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
SMKP03_RS18660 3845238..3845378 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
SMKP03_RS18665 3845378..3845641 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T36750 WP_002892050.1 NZ_AP023148:3841470-3841688 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T36750 NZ_AP023148:3841470-3841688 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT36750 WP_002892066.1 NZ_AP023148:3841069-3841443 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT36750 NZ_AP023148:3841069-3841443 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References