Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3235226..3235862 Replicon chromosome
Accession NZ_AP023089
Organism Bacillus paralicheniformis strain RSC-2

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag KI219_RS16060 Protein ID WP_003179128.1
Coordinates 3235512..3235862 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag KI219_RS16055 Protein ID WP_006638778.1
Coordinates 3235226..3235507 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI219_RS16035 (RSC2_03310) 3230337..3231821 + 1485 WP_048349918.1 PH domain-containing protein -
KI219_RS16040 (RSC2_03311) 3231818..3232417 - 600 WP_075213088.1 rhomboid family intramembrane serine protease -
KI219_RS16045 (RSC2_03312) 3232761..3233720 + 960 WP_164468292.1 outer membrane lipoprotein carrier protein LolA -
KI219_RS16050 (RSC2_03313) 3233945..3235114 + 1170 WP_048349920.1 alanine racemase -
KI219_RS16055 (RSC2_03314) 3235226..3235507 + 282 WP_006638778.1 hypothetical protein Antitoxin
KI219_RS16060 (RSC2_03315) 3235512..3235862 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KI219_RS16065 (RSC2_03316) 3235980..3236807 + 828 WP_023857863.1 RsbT co-antagonist protein RsbRA -
KI219_RS16070 (RSC2_03317) 3236811..3237176 + 366 WP_031305225.1 STAS domain-containing protein -
KI219_RS16075 (RSC2_03318) 3237179..3237580 + 402 WP_023857861.1 anti-sigma regulatory factor -
KI219_RS16080 (RSC2_03319) 3237591..3238598 + 1008 WP_020450261.1 PP2C family protein-serine/threonine phosphatase -
KI219_RS16085 (RSC2_03320) 3238657..3238983 + 327 WP_020450262.1 anti-sigma factor antagonist -
KI219_RS16090 (RSC2_03321) 3238983..3239465 + 483 WP_020450263.1 anti-sigma B factor RsbW -
KI219_RS16095 (RSC2_03322) 3239431..3240222 + 792 WP_023857858.1 RNA polymerase sigma factor SigB -
KI219_RS16100 (RSC2_03323) 3240219..3240818 + 600 WP_035337155.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T36737 WP_003179128.1 NZ_AP023089:3235512-3235862 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T36737 NZ_AP023089:3235512-3235862 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT36737 WP_006638778.1 NZ_AP023089:3235226-3235507 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT36737 NZ_AP023089:3235226-3235507 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References