Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 3235226..3235862 | Replicon | chromosome |
| Accession | NZ_AP023089 | ||
| Organism | Bacillus paralicheniformis strain RSC-2 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | M5P3Q9 |
| Locus tag | KI219_RS16060 | Protein ID | WP_003179128.1 |
| Coordinates | 3235512..3235862 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | M5PDU2 |
| Locus tag | KI219_RS16055 | Protein ID | WP_006638778.1 |
| Coordinates | 3235226..3235507 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KI219_RS16035 (RSC2_03310) | 3230337..3231821 | + | 1485 | WP_048349918.1 | PH domain-containing protein | - |
| KI219_RS16040 (RSC2_03311) | 3231818..3232417 | - | 600 | WP_075213088.1 | rhomboid family intramembrane serine protease | - |
| KI219_RS16045 (RSC2_03312) | 3232761..3233720 | + | 960 | WP_164468292.1 | outer membrane lipoprotein carrier protein LolA | - |
| KI219_RS16050 (RSC2_03313) | 3233945..3235114 | + | 1170 | WP_048349920.1 | alanine racemase | - |
| KI219_RS16055 (RSC2_03314) | 3235226..3235507 | + | 282 | WP_006638778.1 | hypothetical protein | Antitoxin |
| KI219_RS16060 (RSC2_03315) | 3235512..3235862 | + | 351 | WP_003179128.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| KI219_RS16065 (RSC2_03316) | 3235980..3236807 | + | 828 | WP_023857863.1 | RsbT co-antagonist protein RsbRA | - |
| KI219_RS16070 (RSC2_03317) | 3236811..3237176 | + | 366 | WP_031305225.1 | STAS domain-containing protein | - |
| KI219_RS16075 (RSC2_03318) | 3237179..3237580 | + | 402 | WP_023857861.1 | anti-sigma regulatory factor | - |
| KI219_RS16080 (RSC2_03319) | 3237591..3238598 | + | 1008 | WP_020450261.1 | PP2C family protein-serine/threonine phosphatase | - |
| KI219_RS16085 (RSC2_03320) | 3238657..3238983 | + | 327 | WP_020450262.1 | anti-sigma factor antagonist | - |
| KI219_RS16090 (RSC2_03321) | 3238983..3239465 | + | 483 | WP_020450263.1 | anti-sigma B factor RsbW | - |
| KI219_RS16095 (RSC2_03322) | 3239431..3240222 | + | 792 | WP_023857858.1 | RNA polymerase sigma factor SigB | - |
| KI219_RS16100 (RSC2_03323) | 3240219..3240818 | + | 600 | WP_035337155.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12990.03 Da Isoelectric Point: 5.7234
>T36737 WP_003179128.1 NZ_AP023089:3235512-3235862 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF
Download Length: 351 bp
>T36737 NZ_AP023089:3235512-3235862 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10649.03 Da Isoelectric Point: 4.6920
>AT36737 WP_006638778.1 NZ_AP023089:3235226-3235507 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG
Download Length: 282 bp
>AT36737 NZ_AP023089:3235226-3235507 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6I7FHI4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | M5PDU2 |