Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 1486202..1486838 | Replicon | chromosome |
| Accession | NZ_AP023088 | ||
| Organism | Bacillus paralicheniformis strain RSC-1 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | M5P3Q9 |
| Locus tag | KI220_RS07350 | Protein ID | WP_003179128.1 |
| Coordinates | 1486488..1486838 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | M5PDU2 |
| Locus tag | KI220_RS07345 | Protein ID | WP_006638778.1 |
| Coordinates | 1486202..1486483 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KI220_RS07325 (RSC1_01459) | 1481313..1482797 | + | 1485 | WP_048349918.1 | PH domain-containing protein | - |
| KI220_RS07330 (RSC1_01460) | 1482794..1483393 | - | 600 | WP_075213088.1 | rhomboid family intramembrane serine protease | - |
| KI220_RS07335 (RSC1_01461) | 1483737..1484696 | + | 960 | WP_164468292.1 | outer membrane lipoprotein carrier protein LolA | - |
| KI220_RS07340 (RSC1_01462) | 1484921..1486090 | + | 1170 | WP_048349920.1 | alanine racemase | - |
| KI220_RS07345 (RSC1_01463) | 1486202..1486483 | + | 282 | WP_006638778.1 | hypothetical protein | Antitoxin |
| KI220_RS07350 (RSC1_01464) | 1486488..1486838 | + | 351 | WP_003179128.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| KI220_RS07355 (RSC1_01465) | 1486956..1487783 | + | 828 | WP_023857863.1 | RsbT co-antagonist protein RsbRA | - |
| KI220_RS07360 (RSC1_01466) | 1487787..1488152 | + | 366 | WP_031305225.1 | STAS domain-containing protein | - |
| KI220_RS07365 (RSC1_01467) | 1488155..1488556 | + | 402 | WP_023857861.1 | anti-sigma regulatory factor | - |
| KI220_RS07370 (RSC1_01468) | 1488567..1489574 | + | 1008 | WP_020450261.1 | PP2C family protein-serine/threonine phosphatase | - |
| KI220_RS07375 (RSC1_01469) | 1489633..1489959 | + | 327 | WP_020450262.1 | anti-sigma factor antagonist | - |
| KI220_RS07380 (RSC1_01470) | 1489959..1490441 | + | 483 | WP_020450263.1 | anti-sigma B factor RsbW | - |
| KI220_RS07385 (RSC1_01471) | 1490407..1491198 | + | 792 | WP_023857858.1 | RNA polymerase sigma factor SigB | - |
| KI220_RS07390 (RSC1_01472) | 1491195..1491794 | + | 600 | WP_035337155.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12990.03 Da Isoelectric Point: 5.7234
>T36735 WP_003179128.1 NZ_AP023088:1486488-1486838 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF
Download Length: 351 bp
>T36735 NZ_AP023088:1486488-1486838 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10649.03 Da Isoelectric Point: 4.6920
>AT36735 WP_006638778.1 NZ_AP023088:1486202-1486483 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG
Download Length: 282 bp
>AT36735 NZ_AP023088:1486202-1486483 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6I7FHI4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | M5PDU2 |