Detailed information of TA system TA036727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/-
• Location: 258748..259357
• Replicon: chromosome
• Accession: NZ_AP023081
• Organism: Pseudomonas solani strain Sm006

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: PSm6_RS01240
• Protein ID: WP_043244951.1
• Coordinates: 259016..259357 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: PSm6_RS01235
• Protein ID: WP_021222697.1
• Coordinates: 258748..259032 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSm6_RS01215 (PSm6_02380)	254151..255449	-	1299	WP_265170487.1	cobyrinate a,c-diamide synthase	-
PSm6_RS01220 (PSm6_02390)	255446..256057	-	612	WP_021222700.1	cob(I)yrinic acid a,c-diamide adenosyltransferase	-
PSm6_RS01225 (PSm6_02400)	256069..257904	-	1836	WP_265170488.1	TonB-dependent vitamin B12 receptor	-
PSm6_RS01230 (PSm6_02420)	258317..258652	+	336	WP_265169306.1	four-helix bundle copper-binding protein	-
PSm6_RS01235 (PSm6_02430)	258748..259032	+	285	WP_021222697.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PSm6_RS01240 (PSm6_02440)	259016..259357	+	342	WP_043244951.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSm6_RS01245 (PSm6_02450)	259372..259692	-	321	WP_021222695.1	hypothetical protein	-
PSm6_RS01250 (PSm6_02460)	259697..260359	-	663	WP_043244953.1	class I SAM-dependent methyltransferase	-
PSm6_RS01255 (PSm6_02470)	260368..260895	-	528	WP_265169307.1	C40 family peptidase	-
PSm6_RS01260 (PSm6_02480)	261070..261681	-	612	WP_031289040.1	C40 family peptidase	-
PSm6_RS01265 (PSm6_02490)	261748..262521	-	774	WP_043244954.1	NAD-dependent deacylase	-
PSm6_RS01270 (PSm6_02500)	262548..263219	-	672	WP_021222690.1	DNA-3-methyladenine glycosylase I	-
PSm6_RS01275 (PSm6_02510)	263306..264130	-	825	WP_021222637.1	tRNA 2-thiocytidine(32) synthetase TtcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-101)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12817.85 Da
• Isoelectric Point: 6.2131

>T36727 WP_043244951.1 NZ_AP023081:259016-259357 [Pseudomonas solani]
MSRKIEVAYATTAEESLVSQIHHLTPFIGAEPAYAKLAALITTSEHHLQAHPLAAPICEEAALLGVRGYRELHIDEFRIL
YRFDETASLVMVALILRQRQSIEEQLINYCLMR

Nucleotide

• Length: 342 bp

>T36727 NZ_AP023081:259016-259357 [Pseudomonas solani]
ATGAGCAGGAAGATTGAGGTCGCCTATGCGACCACCGCCGAAGAAAGCCTGGTTTCGCAGATCCACCACCTGACGCCTTT
CATTGGCGCCGAACCGGCCTACGCCAAGCTCGCAGCCCTGATCACAACCTCCGAGCACCATCTGCAAGCCCACCCACTGG
CCGCCCCCATCTGCGAGGAAGCCGCCTTGCTGGGCGTCCGCGGCTACCGCGAACTGCATATCGATGAGTTCCGCATCCTC
TATCGCTTCGACGAGACCGCCAGCCTGGTGATGGTCGCGCTGATCCTGCGGCAGCGACAGAGCATCGAAGAGCAATTGAT
CAACTACTGCCTGATGCGTTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10613.07 Da
• Isoelectric Point: 5.8768

>AT36727 WP_021222697.1 NZ_AP023081:258748-259032 [Pseudomonas solani]
MKVRTISYLKQHAASLDLSEPMVITQNGVPAYVVESYAERRRRDEALALIKLLSFAERDKAEGRLISADTLLQRLKQRSP
LTQGPGSIEDEQED

Nucleotide

• Length: 285 bp

>AT36727 NZ_AP023081:258748-259032 [Pseudomonas solani]
ATGAAAGTCCGGACCATCAGCTACCTCAAACAGCACGCCGCCTCGCTGGACCTGAGCGAGCCCATGGTGATCACCCAGAA
CGGCGTACCGGCCTACGTCGTGGAATCCTATGCCGAACGCCGCCGCCGCGATGAAGCCCTGGCGCTGATCAAGCTGCTGT
CGTTCGCGGAACGGGACAAGGCAGAGGGCCGCCTCATCTCCGCCGACACCCTGCTGCAGCGCCTCAAACAGCGCAGCCCC
CTCACCCAAGGACCGGGAAGCATCGAGGATGAGCAGGAAGATTGA