Detailed information of TA system TA036725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 7240..7828
• Replicon: plasmid pOCU_Ac16a_3
• Accession: NZ_AP023080
• Organism: Acinetobacter baumannii strain OCU_Ac16a

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: OCUAC_RS19815
• Protein ID: WP_000438825.1
• Coordinates: 7240..7527 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: OCUAC_RS19820
• Protein ID: WP_004728120.1
• Coordinates: 7514..7828 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCUAC_RS19775	2330..2959	-	630	WP_072689799.1	hypothetical protein	-
OCUAC_RS19780	3171..3539	+	369	WP_017399704.1	hypothetical protein	-
OCUAC_RS19785	3599..4111	-	513	WP_072689801.1	YfbU family protein	-
OCUAC_RS19790	4264..4875	-	612	WP_000571899.1	recombinase family protein	-
OCUAC_RS19795	5056..5583	-	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
OCUAC_RS19800	5699..5989	-	291	WP_005093398.1	putative addiction module antidote protein	-
OCUAC_RS19805	5991..6287	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	-
OCUAC_RS19810	6441..6971	+	531	Protein_9	hypothetical protein	-
OCUAC_RS19815	7240..7527	+	288	WP_000438825.1	BrnT family toxin	Toxin
OCUAC_RS19820	7514..7828	+	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
OCUAC_RS19825	7878..8484	-	607	Protein_12	LysE family translocator	-
OCUAC_RS19830	8534..9362	-	829	Protein_13	AraC family transcriptional regulator	-
OCUAC_RS19835	9490..10242	-	753	Protein_14	IS1-like element ISAba3 family transposase	-
OCUAC_RS19840	10333..11175	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
OCUAC_RS19845	11368..12072	-	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
OCUAC_RS19850	12137..12619	+	483	Protein_17	plasmid mobilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58	-	1..13096	13096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T36725 WP_000438825.1 NZ_AP023080:7240-7527 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T36725 NZ_AP023080:7240-7527 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT36725 WP_004728120.1 NZ_AP023080:7514-7828 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT36725 NZ_AP023080:7514-7828 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9