Detailed information of TA system TA036724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 5699..6287
• Replicon: plasmid pOCU_Ac16a_3
• Accession: NZ_AP023080
• Organism: Acinetobacter baumannii strain OCU_Ac16a

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: OCUAC_RS19805
• Protein ID: WP_004647749.1
• Coordinates: 5991..6287 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3B7PNW4
• Locus tag: OCUAC_RS19800
• Protein ID: WP_005093398.1
• Coordinates: 5699..5989 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCUAC_RS19770	1614..2141	+	528	WP_057073161.1	plasmid replication DNA-binding protein	-
OCUAC_RS19775	2330..2959	-	630	WP_072689799.1	hypothetical protein	-
OCUAC_RS19780	3171..3539	+	369	WP_017399704.1	hypothetical protein	-
OCUAC_RS19785	3599..4111	-	513	WP_072689801.1	YfbU family protein	-
OCUAC_RS19790	4264..4875	-	612	WP_000571899.1	recombinase family protein	-
OCUAC_RS19795	5056..5583	-	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
OCUAC_RS19800	5699..5989	-	291	WP_005093398.1	putative addiction module antidote protein	Antitoxin
OCUAC_RS19805	5991..6287	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCUAC_RS19810	6441..6971	+	531	Protein_9	hypothetical protein	-
OCUAC_RS19815	7240..7527	+	288	WP_000438825.1	BrnT family toxin	-
OCUAC_RS19820	7514..7828	+	315	WP_004728120.1	BrnA antitoxin family protein	-
OCUAC_RS19825	7878..8484	-	607	Protein_12	LysE family translocator	-
OCUAC_RS19830	8534..9362	-	829	Protein_13	AraC family transcriptional regulator	-
OCUAC_RS19835	9490..10242	-	753	Protein_14	IS1-like element ISAba3 family transposase	-
OCUAC_RS19840	10333..11175	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58	-	1..13096	13096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (7-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T36724 WP_004647749.1 NZ_AP023080:c6287-5991 [Acinetobacter baumannii]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T36724 NZ_AP023080:c6287-5991 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTTCCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGTATCCGCCTGAA
TCGTCGATTAGATAAGGTTCAACGTGGAAACTGGGGAGACATTAAGCCTCTCCAAGATGGCGTTTGGGAAATGAGAGAAT
TCTTCGGTTCTGGATGGAGGATGTATTACATCCAACATGGTGACGTTGTGATTGTAATGCTTGGTGGTGGAGATAAATCA
ACTCAACAACAAGACATCGATCGTGCAGTCAAACTATCGAAAACGTTGGAGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10533.20 Da
• Isoelectric Point: 5.6158

>AT36724 WP_005093398.1 NZ_AP023080:c5989-5699 [Acinetobacter baumannii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRHDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT36724 NZ_AP023080:c5989-5699 [Acinetobacter baumannii]
ATGGTTAAAGTAGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGAGAATGATCCAGCTGAATTAGCTCATGCACTTGGTGTTATTGCCAAAGCTCGTGGCATGACTCAAA
TCGCTAAAGAAGCAGGAATTGGGCGTGAGGCACTCTACAAAGCCCTACGTCATGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTGAATGCTTTAGGCTTAAAACTCACAGTACAACACGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A3B7PNW4