Detailed information of TA system TA036720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 3645109..3645693
• Replicon: chromosome
• Accession: NZ_AP023077
• Organism: Acinetobacter baumannii strain OCU_Ac16a

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: OCUAC_RS17440
• Protein ID: WP_000286964.1
• Coordinates: 3645391..3645693 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: OCUAC_RS17435
• Protein ID: WP_000985609.1
• Coordinates: 3645109..3645390 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCUAC_RS17405	3640176..3640787	+	612	WP_015060246.1	recombinase family protein	-
OCUAC_RS17410	3640977..3641861	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
OCUAC_RS17415	3641917..3643392	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
OCUAC_RS17420	3643885..3644157	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
OCUAC_RS17425	3644150..3644470	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
OCUAC_RS17430	3644610..3645029	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
OCUAC_RS17435	3645109..3645390	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
OCUAC_RS17440	3645391..3645693	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCUAC_RS17445	3645842..3646372	+	531	WP_000172759.1	hypothetical protein	-
OCUAC_RS17450	3646461..3646775	-	315	WP_004728120.1	BrnA antitoxin family protein	-
OCUAC_RS17455	3646762..3647049	-	288	WP_000438825.1	BrnT family toxin	-
OCUAC_RS17460	3647279..3647884	-	606	WP_000447788.1	LysE family translocator	-
OCUAC_RS17465	3647934..3648762	-	829	Protein_3406	AraC family transcriptional regulator	-
OCUAC_RS17470	3648890..3649501	-	612	Protein_3407	IS1-like element ISAba3 family transposase	-
OCUAC_RS17475	3649565..3650269	+	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
OCUAC_RS17480	3650323..3650580	-	258	Protein_3409	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T36720 WP_000286964.1 NZ_AP023077:c3645693-3645391 [Acinetobacter baumannii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T36720 NZ_AP023077:c3645693-3645391 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT36720 WP_000985609.1 NZ_AP023077:c3645390-3645109 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT36720 NZ_AP023077:c3645390-3645109 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94