Detailed information of TA system TA036715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 268..731
• Replicon: plasmid pMTY16691
• Accession: NZ_AP023076
• Organism: Neisseria gonorrhoeae strain TUM16691

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: Q51003
• Locus tag: HT073_RS11385
• Protein ID: WP_003690225.1
• Coordinates: 453..731 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: Q51002
• Locus tag: HT073_RS11380
• Protein ID: WP_003690223.1
• Coordinates: 268..453 (+)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HT073_RS11375	20..265	+	246	WP_003690221.1	hypothetical protein	-
HT073_RS11380	268..453	+	186	WP_003690223.1	DUF5397 family protein	Antitoxin
HT073_RS11385	453..731	+	279	WP_003690225.1	endoribonuclease VapD	Toxin
HT073_RS11390	709..978	-	270	WP_003692166.1	hypothetical protein	-
HT073_RS11395	1010..1597	-	588	WP_003692168.1	B protein	-
HT073_RS11400	1681..2310	-	630	WP_003696452.1	relaxase/mobilization nuclease domain-containing protein	-
HT073_RS11405	2295..2486	-	192	WP_003690218.1	plasmid mobilization relaxosome protein MobC	-
HT073_RS11590	2754..2978	+	225	WP_025455920.1	hypothetical protein	-
HT073_RS11410	3148..3930	+	783	WP_010359605.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4153	4153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (2-60)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 6.9726

>T36715 WP_003690225.1 NZ_AP023076:453-731 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Nucleotide

• Length: 279 bp

>T36715 NZ_AP023076:453-731 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 6717.54 Da
• Isoelectric Point: 4.2641

>AT36715 WP_003690223.1 NZ_AP023076:268-453 [Neisseria gonorrhoeae]
MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESGEHTQYKLSHIVQDPKAA

Nucleotide

• Length: 186 bp

>AT36715 NZ_AP023076:268-453 [Neisseria gonorrhoeae]
ATGCAAACCACTATCCCCACCGGTTCAATCCGCACCTTTGGCGATTACGGCGTGATGTACATCGTCGGCACGCCTGCCGA
ACAGCTTCCGGACGGCGATTGGCTCGTAAATATTGAACTGCCGGAAAGCGGCGAACACACGCAATACAAACTGTCCCACA
TCGTTCAAGACCCAAAGGCTGCCTAA

Structures

Toxin

• AlphaFold DB: A0A7H5FJI5

Antitoxin

• AlphaFold DB: Q51002