Detailed information of TA system TA036706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 599..1062
• Replicon: plasmid pMTY19853
• Accession: NZ_AP023068
• Organism: Neisseria gonorrhoeae strain TUM19853

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: Q51003
• Locus tag: HT079_RS11635
• Protein ID: WP_003690225.1
• Coordinates: 784..1062 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: Q51002
• Locus tag: HT079_RS11630
• Protein ID: WP_003690223.1
• Coordinates: 599..784 (+)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HT079_RS11620	112..354	+	243	WP_003690220.1	HTH domain-containing protein	-
HT079_RS11625	351..596	+	246	WP_003690221.1	hypothetical protein	-
HT079_RS11630	599..784	+	186	WP_003690223.1	DUF5397 family protein	Antitoxin
HT079_RS11635	784..1062	+	279	WP_003690225.1	endoribonuclease VapD	Toxin
HT079_RS11640	1040..1309	-	270	WP_003692166.1	hypothetical protein	-
HT079_RS11645	1341..1982	-	642	WP_003690229.1	Cryptic plasmid protein B	-
HT079_RS11650	2066..2695	-	630	WP_003696452.1	relaxase/mobilization nuclease domain-containing protein	-
HT079_RS11655	2680..2871	-	192	WP_003690218.1	plasmid mobilization relaxosome protein MobC	-
HT079_RS11660	3139..3363	+	225	WP_025455920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4207	4207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (2-60)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 6.9726

>T36706 WP_003690225.1 NZ_AP023068:784-1062 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Nucleotide

• Length: 279 bp

>T36706 NZ_AP023068:784-1062 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 6717.54 Da
• Isoelectric Point: 4.2641

>AT36706 WP_003690223.1 NZ_AP023068:599-784 [Neisseria gonorrhoeae]
MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESGEHTQYKLSHIVQDPKAA

Nucleotide

• Length: 186 bp

>AT36706 NZ_AP023068:599-784 [Neisseria gonorrhoeae]
ATGCAAACCACTATCCCCACCGGTTCAATCCGCACCTTTGGCGATTACGGCGTGATGTACATCGTCGGCACGCCTGCCGA
ACAGCTTCCGGACGGCGATTGGCTCGTAAATATTGAACTGCCGGAAAGCGGCGAACACACGCAATACAAACTGTCCCACA
TCGTTCAAGACCCAAAGGCTGCCTAA

Structures

Toxin

• AlphaFold DB: A0A7H5FJI5

Antitoxin

• AlphaFold DB: Q51002