Detailed information of TA system TA036669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2396843..2397817
• Replicon: chromosome
• Accession: NZ_AP023000
• Organism: Bacillus cereus strain 30077

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L6491_RS12330
• Protein ID: WP_062923173.1
• Coordinates: 2396843..2397580 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L6491_RS12335
• Protein ID: WP_062923165.1
• Coordinates: 2397692..2397817 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6491_RS12305 (BC30077_2388)	2392338..2393039	+	702	WP_236788434.1	hypothetical protein	-
L6491_RS12310 (BC30077_2389)	2393166..2394050	+	885	WP_062923162.1	hypothetical protein	-
L6491_RS12315	2394359..2395161	-	803	Protein_2351	thiamine pyrophosphate-binding protein	-
L6491_RS12320 (BC30077_2392)	2395268..2395750	+	483	WP_062923163.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6491_RS12325 (BC30077_2393)	2395917..2396654	+	738	WP_062923164.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6491_RS12330 (BC30077_2394)	2396843..2397580	+	738	WP_062923173.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6491_RS12335 (BC30077_2395)	2397692..2397817	+	126	WP_062923165.1	hypothetical protein	Antitoxin
L6491_RS12340 (BC30077_2396)	2397893..2398069	+	177	WP_062923166.1	stage II sporulation protein SB	-
L6491_RS12345 (BC30077_2397)	2398088..2398477	-	390	WP_062923167.1	YxeA family protein	-
L6491_RS12350 (BC30077_2398)	2398694..2400163	+	1470	WP_062923168.1	beta-Ala-His dipeptidase	-
L6491_RS12355 (BC30077_2399)	2400745..2401215	-	471	WP_061530815.1	DUF5065 family protein	-
L6491_RS12360 (BC30077_2400)	2401669..2402328	+	660	WP_000876955.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.03 Da
• Isoelectric Point: 8.3034

>T36669 WP_062923173.1 NZ_AP023000:2396843-2397580 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYIKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKTVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T36669 NZ_AP023000:2396843-2397580 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGTTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAGTTGTACGAAGAGAAGAAGCAACGGATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATATAAAAAGCTTAAACGCATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGCTCACAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCGGCAAAAACTGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAATCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT36669 WP_062923165.1 NZ_AP023000:2397692-2397817 [Bacillus cereus]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT36669 NZ_AP023000:2397692-2397817 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGATGAATTAAAAAGAAAATGA