Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 260995..261637 Replicon chromosome
Accession NZ_AP023000
Organism Bacillus cereus strain 30077

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6491_RS01475 Protein ID WP_000635963.1
Coordinates 261287..261637 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6491_RS01470 Protein ID WP_000004570.1
Coordinates 260995..261282 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6491_RS01445 (BC30077_0240) 256311..257273 + 963 WP_236787436.1 UV DNA damage repair endonuclease UvsE -
L6491_RS01450 (BC30077_0241) 257266..257838 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6491_RS01455 (BC30077_0242) 257931..258290 + 360 WP_000635040.1 holo-ACP synthase -
L6491_RS01460 (BC30077_0243) 258447..259397 + 951 WP_236788600.1 outer membrane lipoprotein carrier protein LolA -
L6491_RS01465 (BC30077_0244) 259516..260685 + 1170 WP_000390600.1 alanine racemase -
L6491_RS01470 (BC30077_0246) 260995..261282 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6491_RS01475 (BC30077_0247) 261287..261637 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6491_RS01480 (BC30077_0248) 261705..263873 + 2169 WP_236787437.1 Tex family protein -
L6491_RS01485 263931..264047 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6491_RS01490 (BC30077_0249) 264243..264701 + 459 WP_062923611.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36668 WP_000635963.1 NZ_AP023000:261287-261637 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36668 NZ_AP023000:261287-261637 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36668 WP_000004570.1 NZ_AP023000:260995-261282 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36668 NZ_AP023000:260995-261282 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References