Detailed information of TA system TA036667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2397130..2398105
• Replicon: chromosome
• Accession: NZ_AP022994
• Organism: Bacillus cereus strain 30075

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L6490_RS12300
• Protein ID: WP_062923173.1
• Coordinates: 2397130..2397867 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L6490_RS12305
• Protein ID: WP_062923165.1
• Coordinates: 2397980..2398105 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6490_RS12275 (BC30075_2393)	2392626..2393327	+	702	WP_236788434.1	hypothetical protein	-
L6490_RS12280 (BC30075_2394)	2393453..2394337	+	885	WP_062923162.1	hypothetical protein	-
L6490_RS12285	2394646..2395448	-	803	Protein_2345	thiamine pyrophosphate-binding protein	-
L6490_RS12290 (BC30075_2397)	2395555..2396037	+	483	WP_062923163.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6490_RS12295 (BC30075_2398)	2396204..2396941	+	738	WP_062923164.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6490_RS12300 (BC30075_2399)	2397130..2397867	+	738	WP_062923173.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6490_RS12305 (BC30075_2400)	2397980..2398105	+	126	WP_062923165.1	hypothetical protein	Antitoxin
L6490_RS12310 (BC30075_2401)	2398181..2398384	+	204	WP_236798343.1	stage II sporulation protein SB	-
L6490_RS12315 (BC30075_2402)	2398374..2398763	-	390	WP_062923167.1	YxeA family protein	-
L6490_RS12320 (BC30075_2403)	2398980..2400449	+	1470	WP_062923168.1	beta-Ala-His dipeptidase	-
L6490_RS12325 (BC30075_2404)	2401031..2401501	-	471	WP_061530815.1	DUF5065 family protein	-
L6490_RS12330 (BC30075_2405)	2401955..2402602	+	648	WP_236798344.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.03 Da
• Isoelectric Point: 8.3034

>T36667 WP_062923173.1 NZ_AP022994:2397130-2397867 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYIKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKTVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T36667 NZ_AP022994:2397130-2397867 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGTTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAGTTGTACGAAGAGAAGAAGCAACGGATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATATAAAAAGCTTAAACGCATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGCTCACAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCGGCAAAAACTGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAATCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT36667 WP_062923165.1 NZ_AP022994:2397980-2398105 [Bacillus cereus]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT36667 NZ_AP022994:2397980-2398105 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGATGAATTAAAAAGAAAATGA