Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 262917..263559 Replicon chromosome
Accession NZ_AP022986
Organism Bacillus cereus strain 30052

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6499_RS01465 Protein ID WP_000635963.1
Coordinates 263209..263559 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6499_RS01460 Protein ID WP_000004570.1
Coordinates 262917..263204 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6499_RS01435 (BC30052_0240) 258233..259195 + 963 WP_000961157.1 UV DNA damage repair endonuclease UvsE -
L6499_RS01440 (BC30052_0241) 259188..259760 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6499_RS01445 (BC30052_0242) 259853..260212 + 360 WP_000635040.1 holo-ACP synthase -
L6499_RS01450 (BC30052_0243) 260369..261319 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
L6499_RS01455 (BC30052_0244) 261438..262607 + 1170 WP_000390600.1 alanine racemase -
L6499_RS01460 (BC30052_0245) 262917..263204 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6499_RS01465 (BC30052_0246) 263209..263559 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6499_RS01470 (BC30052_0247) 263627..265795 + 2169 WP_000426225.1 Tex family protein -
L6499_RS01475 265853..265969 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6499_RS01480 (BC30052_0248) 266165..266623 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36664 WP_000635963.1 NZ_AP022986:263209-263559 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36664 NZ_AP022986:263209-263559 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36664 WP_000004570.1 NZ_AP022986:262917-263204 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36664 NZ_AP022986:262917-263204 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References