Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240462..241104 Replicon chromosome
Accession NZ_AP022981
Organism Bacillus cereus strain 30048

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6480_RS01370 Protein ID WP_000635963.1
Coordinates 240754..241104 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6480_RS01365 Protein ID WP_000004570.1
Coordinates 240462..240749 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6480_RS01340 (BC30048_0219) 235779..236741 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
L6480_RS01345 (BC30048_0220) 236734..237306 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
L6480_RS01350 (BC30048_0221) 237400..237759 + 360 WP_000583416.1 holo-ACP synthase -
L6480_RS01355 (BC30048_0222) 237916..238866 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
L6480_RS01360 (BC30048_0223) 238984..240153 + 1170 WP_000390615.1 alanine racemase -
L6480_RS01365 (BC30048_0224) 240462..240749 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6480_RS01370 (BC30048_0225) 240754..241104 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6480_RS01375 (BC30048_0226) 241172..243340 + 2169 WP_000426242.1 Tex family protein -
L6480_RS01380 243398..243514 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6480_RS01385 (BC30048_0227) 243710..244168 + 459 WP_000344231.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36662 WP_000635963.1 NZ_AP022981:240754-241104 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36662 NZ_AP022981:240754-241104 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36662 WP_000004570.1 NZ_AP022981:240462-240749 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36662 NZ_AP022981:240462-240749 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References