Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 253830..254472 Replicon chromosome
Accession NZ_AP022978
Organism Bacillus cereus strain 30043

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6436_RS01435 Protein ID WP_000635963.1
Coordinates 254122..254472 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6436_RS01430 Protein ID WP_000004570.1
Coordinates 253830..254117 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6436_RS01405 (BC30043_0229) 249146..250108 + 963 WP_236787436.1 UV DNA damage repair endonuclease UvsE -
L6436_RS01410 (BC30043_0230) 250101..250673 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6436_RS01415 (BC30043_0231) 250766..251125 + 360 WP_000635040.1 holo-ACP synthase -
L6436_RS01420 (BC30043_0232) 251282..252232 + 951 WP_236788600.1 outer membrane lipoprotein carrier protein LolA -
L6436_RS01425 (BC30043_0233) 252351..253520 + 1170 WP_000390600.1 alanine racemase -
L6436_RS01430 (BC30043_0235) 253830..254117 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6436_RS01435 (BC30043_0236) 254122..254472 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6436_RS01440 (BC30043_0237) 254540..256708 + 2169 WP_236787437.1 Tex family protein -
L6436_RS01445 256766..256882 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6436_RS01450 (BC30043_0238) 257078..257536 + 459 WP_062923611.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36660 WP_000635963.1 NZ_AP022978:254122-254472 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36660 NZ_AP022978:254122-254472 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36660 WP_000004570.1 NZ_AP022978:253830-254117 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36660 NZ_AP022978:253830-254117 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References