Detailed information of TA system TA036659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2392434..2393409
• Replicon: chromosome
• Accession: NZ_AP022975
• Organism: Bacillus cereus strain 30040

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L6438_RS12275
• Protein ID: WP_062923173.1
• Coordinates: 2392434..2393171 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L6438_RS12280
• Protein ID: WP_062923165.1
• Coordinates: 2393284..2393409 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6438_RS12250 (BC30040_2373)	2387929..2388630	+	702	WP_236788434.1	hypothetical protein	-
L6438_RS12255 (BC30040_2374)	2388757..2389641	+	885	WP_062923162.1	hypothetical protein	-
L6438_RS12260	2389950..2390752	-	803	Protein_2340	thiamine pyrophosphate-binding protein	-
L6438_RS12265 (BC30040_2377)	2390859..2391341	+	483	WP_062923163.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6438_RS12270 (BC30040_2378)	2391508..2392245	+	738	WP_062923164.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6438_RS12275 (BC30040_2379)	2392434..2393171	+	738	WP_062923173.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6438_RS12280 (BC30040_2380)	2393284..2393409	+	126	WP_062923165.1	hypothetical protein	Antitoxin
L6438_RS12285 (BC30040_2381)	2393485..2393661	+	177	WP_062923166.1	stage II sporulation protein SB	-
L6438_RS12290 (BC30040_2382)	2393680..2394069	-	390	WP_062923167.1	YxeA family protein	-
L6438_RS12295 (BC30040_2383)	2394286..2395755	+	1470	WP_062923168.1	beta-Ala-His dipeptidase	-
L6438_RS12300 (BC30040_2384)	2396337..2396807	-	471	WP_061530815.1	DUF5065 family protein	-
L6438_RS12305 (BC30040_2385)	2397261..2397920	+	660	WP_000876955.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.03 Da
• Isoelectric Point: 8.3034

>T36659 WP_062923173.1 NZ_AP022975:2392434-2393171 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYIKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKTVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T36659 NZ_AP022975:2392434-2393171 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGTTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAGTTGTACGAAGAGAAGAAGCAACGGATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATATAAAAAGCTTAAACGCATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGCTCACAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCGGCAAAAACTGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAATCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT36659 WP_062923165.1 NZ_AP022975:2393284-2393409 [Bacillus cereus]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT36659 NZ_AP022975:2393284-2393409 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGATGAATTAAAAAGAAAATGA