Detailed information of TA system TA036657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2383832..2384807
• Replicon: chromosome
• Accession: NZ_AP022970
• Organism: Bacillus cereus strain J75

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L6478_RS12230
• Protein ID: WP_062923173.1
• Coordinates: 2383832..2384569 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L6478_RS12235
• Protein ID: WP_062923165.1
• Coordinates: 2384682..2384807 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6478_RS12205 (BCJMU75_2362)	2379326..2380027	+	702	WP_236788434.1	hypothetical protein	-
L6478_RS12210 (BCJMU75_2363)	2380155..2381039	+	885	WP_062923162.1	hypothetical protein	-
L6478_RS12215	2381348..2382150	-	803	Protein_2329	thiamine pyrophosphate-binding protein	-
L6478_RS12220 (BCJMU75_2366)	2382257..2382739	+	483	WP_062923163.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6478_RS12225 (BCJMU75_2367)	2382906..2383643	+	738	WP_062923164.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6478_RS12230 (BCJMU75_2368)	2383832..2384569	+	738	WP_062923173.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6478_RS12235 (BCJMU75_2369)	2384682..2384807	+	126	WP_062923165.1	hypothetical protein	Antitoxin
L6478_RS12240 (BCJMU75_2370)	2384883..2385059	+	177	WP_062923166.1	stage II sporulation protein SB	-
L6478_RS12245 (BCJMU75_2371)	2385078..2385467	-	390	WP_062923167.1	YxeA family protein	-
L6478_RS12250 (BCJMU75_2372)	2385684..2387153	+	1470	WP_062923168.1	beta-Ala-His dipeptidase	-
L6478_RS12255 (BCJMU75_2373)	2387735..2388205	-	471	WP_236795912.1	DUF5065 family protein	-
L6478_RS12260 (BCJMU75_2374)	2388659..2389318	+	660	WP_000876955.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.03 Da
• Isoelectric Point: 8.3034

>T36657 WP_062923173.1 NZ_AP022970:2383832-2384569 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYIKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKTVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T36657 NZ_AP022970:2383832-2384569 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGTTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAGTTGTACGAAGAGAAGAAGCAACGGATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATATAAAAAGCTTAAACGCATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGCTCACAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCGGCAAAAACTGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAATCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT36657 WP_062923165.1 NZ_AP022970:2384682-2384807 [Bacillus cereus]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT36657 NZ_AP022970:2384682-2384807 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGATGAATTAAAAAGAAAATGA