Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 265499..266141 Replicon chromosome
Accession NZ_AP022964
Organism Bacillus cereus strain J62

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6484_RS01470 Protein ID WP_000635963.1
Coordinates 265791..266141 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6484_RS01465 Protein ID WP_000004570.1
Coordinates 265499..265786 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6484_RS01440 (BCJMU62_0245) 260815..261777 + 963 WP_000961157.1 UV DNA damage repair endonuclease UvsE -
L6484_RS01445 (BCJMU62_0246) 261770..262342 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6484_RS01450 (BCJMU62_0247) 262435..262794 + 360 WP_000635040.1 holo-ACP synthase -
L6484_RS01455 (BCJMU62_0248) 262951..263901 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
L6484_RS01460 (BCJMU62_0249) 264020..265189 + 1170 WP_000390600.1 alanine racemase -
L6484_RS01465 (BCJMU62_0250) 265499..265786 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6484_RS01470 (BCJMU62_0251) 265791..266141 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6484_RS01475 (BCJMU62_0252) 266209..268377 + 2169 WP_200152485.1 Tex family protein -
L6484_RS01480 268435..268551 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6484_RS01485 (BCJMU62_0253) 268747..269205 + 459 WP_078384249.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36654 WP_000635963.1 NZ_AP022964:265791-266141 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36654 NZ_AP022964:265791-266141 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36654 WP_000004570.1 NZ_AP022964:265499-265786 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36654 NZ_AP022964:265499-265786 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References