Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2363507..2364485 Replicon chromosome
Accession NZ_AP022946
Organism Bacillus cereus strain J10

Toxin (Protein)


Gene name spoIISA Uniprot ID W8Y388
Locus tag L6486_RS12170 Protein ID WP_000624977.1
Coordinates 2363507..2364244 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID W8YZL5
Locus tag L6486_RS12175 Protein ID WP_000237818.1
Coordinates 2364357..2364485 (+) Length 43 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6486_RS12150 (BCJMU10_2368) 2359149..2359931 + 783 WP_063219931.1 class I SAM-dependent methyltransferase -
L6486_RS12155 (BCJMU10_2369) 2360089..2361774 - 1686 WP_063219932.1 alpha-keto acid decarboxylase family protein -
L6486_RS12160 (BCJMU10_2370) 2361882..2362364 + 483 WP_000191886.1 MarR family winged helix-turn-helix transcriptional regulator -
L6486_RS12165 (BCJMU10_2371) 2362531..2363268 + 738 WP_000594152.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
L6486_RS12170 (BCJMU10_2372) 2363507..2364244 + 738 WP_000624977.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
L6486_RS12175 (BCJMU10_2373) 2364357..2364485 + 129 WP_000237818.1 hypothetical protein Antitoxin
L6486_RS12180 (BCJMU10_2374) 2364556..2364732 + 177 WP_000852629.1 stage II sporulation protein SB -
L6486_RS12185 (BCJMU10_2375) 2364749..2365138 - 390 WP_000713862.1 YxeA family protein -
L6486_RS12190 (BCJMU10_2376) 2365350..2366819 + 1470 WP_063219933.1 beta-Ala-His dipeptidase -
L6486_RS12195 (BCJMU10_2377) 2367065..2367874 + 810 WP_063219934.1 papain-like cysteine protease family protein -
L6486_RS12200 (BCJMU10_2378) 2367900..2368502 + 603 WP_000517271.1 hypothetical protein -
L6486_RS12205 (BCJMU10_2379) 2368746..2368997 - 252 WP_000827560.1 LuxR C-terminal-related transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28307.03 Da        Isoelectric Point: 8.2998

>T36649 WP_000624977.1 NZ_AP022946:2363507-2364244 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T36649 NZ_AP022946:2363507-2364244 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 43 a.a.        Molecular weight: 5011.40 Da        Isoelectric Point: 3.9150

>AT36649 WP_000237818.1 NZ_AP022946:2364357-2364485 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Download         Length: 129 bp

>AT36649 NZ_AP022946:2364357-2364485 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W8Y388

Antitoxin

Source ID Structure
AlphaFold DB W8YZL5

References