Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240399..241041 Replicon chromosome
Accession NZ_AP022946
Organism Bacillus cereus strain J10

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6486_RS01370 Protein ID WP_000635963.1
Coordinates 240691..241041 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6486_RS01365 Protein ID WP_000004570.1
Coordinates 240399..240686 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6486_RS01340 (BCJMU10_0221) 235716..236678 + 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -
L6486_RS01345 (BCJMU10_0222) 236671..237243 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
L6486_RS01350 (BCJMU10_0223) 237337..237696 + 360 WP_063219903.1 holo-ACP synthase -
L6486_RS01355 (BCJMU10_0224) 237853..238803 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
L6486_RS01360 (BCJMU10_0225) 238921..240090 + 1170 WP_000390615.1 alanine racemase -
L6486_RS01365 (BCJMU10_0226) 240399..240686 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6486_RS01370 (BCJMU10_0227) 240691..241041 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6486_RS01375 (BCJMU10_0228) 241109..243277 + 2169 WP_000426242.1 Tex family protein -
L6486_RS01380 243335..243451 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6486_RS01385 (BCJMU10_0229) 243647..244105 + 459 WP_000344234.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36648 WP_000635963.1 NZ_AP022946:240691-241041 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36648 NZ_AP022946:240691-241041 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36648 WP_000004570.1 NZ_AP022946:240399-240686 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36648 NZ_AP022946:240399-240686 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References