36647

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 261404..262046 Replicon chromosome
Accession NZ_AP022934
Organism Bacillus cereus strain J7

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6482_RS01460 Protein ID WP_000635963.1
Coordinates 261696..262046 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6482_RS01455 Protein ID WP_000004570.1
Coordinates 261404..261691 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6482_RS01430 (BCJMU07_0238) 256720..257682 + 963 WP_000961157.1 UV DNA damage repair endonuclease UvsE -
L6482_RS01435 (BCJMU07_0239) 257675..258247 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6482_RS01440 (BCJMU07_0240) 258340..258699 + 360 WP_000635040.1 holo-ACP synthase -
L6482_RS01445 (BCJMU07_0241) 258856..259806 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
L6482_RS01450 (BCJMU07_0242) 259925..261094 + 1170 WP_000390600.1 alanine racemase -
L6482_RS01455 (BCJMU07_0243) 261404..261691 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6482_RS01460 (BCJMU07_0244) 261696..262046 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6482_RS01465 (BCJMU07_0245) 262114..264282 + 2169 WP_200152485.1 Tex family protein -
L6482_RS01470 264340..264456 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6482_RS01475 (BCJMU07_0246) 264652..265110 + 459 WP_078384249.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36647 WP_000635963.1 NZ_AP022934:261696-262046 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36647 NZ_AP022934:261696-262046 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36647 WP_000004570.1 NZ_AP022934:261404-261691 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36647 NZ_AP022934:261404-261691 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References