Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 249817..250459 Replicon chromosome
Accession NZ_AP022927
Organism Bacillus cereus strain J2

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6497_RS01420 Protein ID WP_000635963.1
Coordinates 250109..250459 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6497_RS01415 Protein ID WP_000004570.1
Coordinates 249817..250104 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6497_RS01390 (BCJMU02_0230) 245133..246095 + 963 WP_000961157.1 UV DNA damage repair endonuclease UvsE -
L6497_RS01395 (BCJMU02_0231) 246088..246660 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6497_RS01400 (BCJMU02_0232) 246753..247112 + 360 WP_000635040.1 holo-ACP synthase -
L6497_RS01405 (BCJMU02_0233) 247269..248219 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
L6497_RS01410 (BCJMU02_0234) 248338..249507 + 1170 WP_236793105.1 alanine racemase -
L6497_RS01415 (BCJMU02_0235) 249817..250104 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6497_RS01420 (BCJMU02_0236) 250109..250459 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6497_RS01425 (BCJMU02_0237) 250527..252695 + 2169 WP_000426222.1 Tex family protein -
L6497_RS01430 252753..252869 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6497_RS01435 (BCJMU02_0238) 253065..253523 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36645 WP_000635963.1 NZ_AP022927:250109-250459 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36645 NZ_AP022927:250109-250459 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36645 WP_000004570.1 NZ_AP022927:249817-250104 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36645 NZ_AP022927:249817-250104 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References