Detailed information of TA system TA036642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2430367..2431342
• Replicon: chromosome
• Accession: NZ_AP022915
• Organism: Bacillus cereus strain MRY14-0105

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L6451_RS12450
• Protein ID: WP_062923173.1
• Coordinates: 2430367..2431104 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L6451_RS12455
• Protein ID: WP_062923165.1
• Coordinates: 2431217..2431342 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6451_RS12425 (BCM0105_2413)	2425861..2426562	+	702	WP_236788434.1	hypothetical protein	-
L6451_RS12430 (BCM0105_2414)	2426690..2427574	+	885	WP_062923162.1	hypothetical protein	-
L6451_RS12435	2427883..2428685	-	803	Protein_2373	thiamine pyrophosphate-binding protein	-
L6451_RS12440 (BCM0105_2417)	2428792..2429274	+	483	WP_062923163.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6451_RS12445 (BCM0105_2418)	2429441..2430178	+	738	WP_062923164.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6451_RS12450 (BCM0105_2419)	2430367..2431104	+	738	WP_062923173.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6451_RS12455 (BCM0105_2420)	2431217..2431342	+	126	WP_062923165.1	hypothetical protein	Antitoxin
L6451_RS12460 (BCM0105_2421)	2431418..2431594	+	177	WP_062923166.1	stage II sporulation protein SB	-
L6451_RS12465 (BCM0105_2422)	2431613..2432002	-	390	WP_062923167.1	YxeA family protein	-
L6451_RS12470 (BCM0105_2423)	2432219..2433688	+	1470	WP_062923168.1	beta-Ala-His dipeptidase	-
L6451_RS12475 (BCM0105_2424)	2434270..2434740	-	471	WP_061530815.1	DUF5065 family protein	-
L6451_RS12480 (BCM0105_2425)	2435194..2435853	+	660	WP_000876955.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.03 Da
• Isoelectric Point: 8.3034

>T36642 WP_062923173.1 NZ_AP022915:2430367-2431104 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYIKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKTVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T36642 NZ_AP022915:2430367-2431104 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGTTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAGTTGTACGAAGAGAAGAAGCAACGGATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATATAAAAAGCTTAAACGCATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGCTCACAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCGGCAAAAACTGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAATCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT36642 WP_062923165.1 NZ_AP022915:2431217-2431342 [Bacillus cereus]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT36642 NZ_AP022915:2431217-2431342 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGATGAATTAAAAAGAAAATGA