Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 261062..261704 Replicon chromosome
Accession NZ_AP022915
Organism Bacillus cereus strain MRY14-0105

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6451_RS01475 Protein ID WP_000635963.1
Coordinates 261354..261704 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6451_RS01470 Protein ID WP_000004570.1
Coordinates 261062..261349 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6451_RS01445 (BCM0105_0240) 256378..257340 + 963 WP_236787436.1 UV DNA damage repair endonuclease UvsE -
L6451_RS01450 (BCM0105_0241) 257333..257905 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6451_RS01455 (BCM0105_0242) 257998..258357 + 360 WP_000635040.1 holo-ACP synthase -
L6451_RS01460 (BCM0105_0243) 258514..259464 + 951 WP_236788600.1 outer membrane lipoprotein carrier protein LolA -
L6451_RS01465 (BCM0105_0244) 259583..260752 + 1170 WP_000390600.1 alanine racemase -
L6451_RS01470 (BCM0105_0246) 261062..261349 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6451_RS01475 (BCM0105_0247) 261354..261704 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6451_RS01480 (BCM0105_0248) 261772..263940 + 2169 WP_236787437.1 Tex family protein -
L6451_RS01485 263998..264114 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6451_RS01490 (BCM0105_0249) 264310..264768 + 459 WP_062923611.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36641 WP_000635963.1 NZ_AP022915:261354-261704 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36641 NZ_AP022915:261354-261704 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36641 WP_000004570.1 NZ_AP022915:261062-261349 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36641 NZ_AP022915:261062-261349 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References