36639

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 248457..249099 Replicon chromosome
Accession NZ_AP022907
Organism Bacillus cereus strain MRY14-0100

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6498_RS01410 Protein ID WP_000635963.1
Coordinates 248749..249099 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6498_RS01405 Protein ID WP_000004570.1
Coordinates 248457..248744 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6498_RS01380 (BCM0100_0227) 243773..244735 + 963 WP_000961157.1 UV DNA damage repair endonuclease UvsE -
L6498_RS01385 (BCM0100_0228) 244728..245300 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6498_RS01390 (BCM0100_0229) 245393..245752 + 360 WP_000635040.1 holo-ACP synthase -
L6498_RS01395 (BCM0100_0230) 245909..246859 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
L6498_RS01400 (BCM0100_0231) 246978..248147 + 1170 WP_000390600.1 alanine racemase -
L6498_RS01405 (BCM0100_0232) 248457..248744 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6498_RS01410 (BCM0100_0233) 248749..249099 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6498_RS01415 (BCM0100_0234) 249167..251335 + 2169 WP_000426225.1 Tex family protein -
L6498_RS01420 251393..251509 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6498_RS01425 (BCM0100_0235) 251705..252163 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36639 WP_000635963.1 NZ_AP022907:248749-249099 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36639 NZ_AP022907:248749-249099 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36639 WP_000004570.1 NZ_AP022907:248457-248744 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36639 NZ_AP022907:248457-248744 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References