Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 251403..252045 Replicon chromosome
Accession NZ_AP022877
Organism Bacillus cereus strain MRY14-0060

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6481_RS01415 Protein ID WP_000635963.1
Coordinates 251695..252045 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6481_RS01410 Protein ID WP_000004570.1
Coordinates 251403..251690 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6481_RS01385 (BCM0060_0228) 246719..247681 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
L6481_RS01390 (BCM0060_0229) 247674..248246 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6481_RS01395 (BCM0060_0230) 248339..248698 + 360 WP_000635040.1 holo-ACP synthase -
L6481_RS01400 (BCM0060_0231) 248855..249805 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
L6481_RS01405 (BCM0060_0232) 249924..251093 + 1170 WP_000390600.1 alanine racemase -
L6481_RS01410 (BCM0060_0233) 251403..251690 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6481_RS01415 (BCM0060_0234) 251695..252045 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6481_RS01420 (BCM0060_0235) 252113..254281 + 2169 WP_236806876.1 Tex family protein -
L6481_RS01425 254339..254455 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6481_RS01430 (BCM0060_0236) 254651..255109 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36633 WP_000635963.1 NZ_AP022877:251695-252045 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36633 NZ_AP022877:251695-252045 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36633 WP_000004570.1 NZ_AP022877:251403-251690 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36633 NZ_AP022877:251403-251690 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References