Detailed information of TA system TA036632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2422159..2423134
• Replicon: chromosome
• Accession: NZ_AP022874
• Organism: Bacillus cereus strain MRY14-0057

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L6489_RS12415
• Protein ID: WP_062923173.1
• Coordinates: 2422159..2422896 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L6489_RS12420
• Protein ID: WP_062923165.1
• Coordinates: 2423009..2423134 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6489_RS12390 (BCM0057_2403)	2417653..2418354	+	702	WP_236788434.1	hypothetical protein	-
L6489_RS12395 (BCM0057_2404)	2418482..2419366	+	885	WP_062923162.1	hypothetical protein	-
L6489_RS12400	2419675..2420477	-	803	Protein_2366	thiamine pyrophosphate-binding protein	-
L6489_RS12405 (BCM0057_2407)	2420584..2421066	+	483	WP_062923163.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6489_RS12410 (BCM0057_2408)	2421233..2421970	+	738	WP_062923164.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6489_RS12415 (BCM0057_2409)	2422159..2422896	+	738	WP_062923173.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6489_RS12420 (BCM0057_2410)	2423009..2423134	+	126	WP_062923165.1	hypothetical protein	Antitoxin
L6489_RS12425 (BCM0057_2411)	2423208..2423384	+	177	WP_062923166.1	stage II sporulation protein SB	-
L6489_RS12430 (BCM0057_2412)	2423403..2423792	-	390	WP_062923167.1	YxeA family protein	-
L6489_RS12435 (BCM0057_2413)	2424009..2425478	+	1470	WP_062923168.1	beta-Ala-His dipeptidase	-
L6489_RS12440 (BCM0057_2414)	2426060..2426530	-	471	WP_061530815.1	DUF5065 family protein	-
L6489_RS12445 (BCM0057_2415)	2426984..2427643	+	660	WP_000876955.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.03 Da
• Isoelectric Point: 8.3034

>T36632 WP_062923173.1 NZ_AP022874:2422159-2422896 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYIKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKTVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T36632 NZ_AP022874:2422159-2422896 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGTTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAGTTGTACGAAGAGAAGAAGCAACGGATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATATAAAAAGCTTAAACGCATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGCTCACAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCGGCAAAAACTGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAATCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT36632 WP_062923165.1 NZ_AP022874:2423009-2423134 [Bacillus cereus]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT36632 NZ_AP022874:2423009-2423134 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGATGAATTAAAAAGAAAATGA