Detailed information of TA system TA036616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3400832..3401354
• Replicon: chromosome
• Accession: NZ_AP022869
• Organism: Halomonas meridiana strain Eplume2

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2M9TBV7
• Locus tag: HMEPL2_RS16160
• Protein ID: WP_058578261.1
• Coordinates: 3400832..3401095 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HMEPL2_RS16165
• Protein ID: WP_172515452.1
• Coordinates: 3401085..3401354 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMEPL2_RS16115	3396015..3396230	+	216	WP_084812740.1	type II toxin-antitoxin system CcdA family antitoxin	-
HMEPL2_RS16120	3396300..3396617	-	318	WP_062373121.1	CcdB family protein	-
HMEPL2_RS16125	3396617..3396862	-	246	WP_172515451.1	type II toxin-antitoxin system CcdA family antitoxin	-
HMEPL2_RS16130	3396986..3397333	+	348	Protein_3160	MBL fold metallo-hydrolase	-
HMEPL2_RS16135	3397362..3398015	+	654	WP_062373116.1	DsbA family oxidoreductase	-
HMEPL2_RS16140	3398166..3398654	+	489	WP_089675732.1	hypothetical protein	-
HMEPL2_RS16145	3398744..3399059	+	316	Protein_3163	aspartate/glutamate racemase family protein	-
HMEPL2_RS16150	3399169..3400280	+	1112	Protein_3164	transposase	-
HMEPL2_RS16155	3400458..3400756	+	299	Protein_3165	amino acid racemase	-
HMEPL2_RS16160	3400832..3401095	+	264	WP_058578261.1	BrnT family toxin	Toxin
HMEPL2_RS16165	3401085..3401354	+	270	WP_172515452.1	CopG family transcriptional regulator	Antitoxin
HMEPL2_RS16170	3401387..3402139	-	753	WP_172515453.1	hypothetical protein	-
HMEPL2_RS16175	3402217..3403389	-	1173	WP_172515454.1	UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase	-
HMEPL2_RS16180	3403486..3404352	+	867	WP_172515455.1	NAD-dependent protein deacetylase	-
HMEPL2_RS16185	3404494..3405987	+	1494	WP_172515456.1	YdiU family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3387248..3403389	16141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10314.77 Da
• Isoelectric Point: 7.2035

>T36616 WP_058578261.1 NZ_AP022869:3400832-3401095 [Halomonas meridiana]
MNEFEFDVNKSQANLTKHGIDFEAAQALWKDPYLLEIRAKSEGEPRFLLIGMISEKHWSAVVTYREERIRLISVRRSRKK
EVDIYES

Nucleotide

• Length: 264 bp

>T36616 NZ_AP022869:3400832-3401095 [Halomonas meridiana]
ATGAACGAGTTCGAGTTTGACGTCAACAAAAGTCAGGCCAACCTGACAAAGCACGGTATTGATTTTGAAGCGGCACAAGC
GCTGTGGAAGGATCCATATCTGCTAGAAATTCGCGCAAAATCCGAGGGCGAACCAAGGTTTTTACTAATCGGTATGATCA
GTGAAAAACATTGGTCGGCAGTCGTCACCTACCGAGAAGAGCGTATTCGGCTGATTTCTGTTAGACGCTCCCGCAAGAAG
GAGGTAGACATCTATGAAAGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10258.50 Da
• Isoelectric Point: 6.7277

>AT36616 WP_172515452.1 NZ_AP022869:3401085-3401354 [Halomonas meridiana]
MKAKDFDKKFDENQDDVLDDLDFSTARRVNQEQKRINVDFPAWVVESLDREAARMGVTRQSIIKVWLVERLQAEAARKPH
GHNSVGGPH

Nucleotide

• Length: 270 bp

>AT36616 NZ_AP022869:3401085-3401354 [Halomonas meridiana]
ATGAAAGCTAAAGATTTCGATAAAAAATTCGATGAAAATCAAGATGATGTTCTCGATGATCTAGACTTTTCCACTGCTCG
ACGGGTGAATCAGGAGCAAAAGCGGATTAACGTAGATTTTCCTGCATGGGTAGTGGAGTCACTAGACCGTGAGGCTGCGA
GAATGGGTGTTACCCGTCAGTCGATTATCAAGGTTTGGTTAGTGGAGCGTCTGCAAGCTGAAGCGGCTCGTAAACCTCAT
GGCCATAACTCAGTAGGGGGACCTCACTAG

Structures

Toxin

• AlphaFold DB: A0A2M9TBV7