Detailed information of TA system TA036597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2424735..2425710
• Replicon: chromosome
• Accession: NZ_AP022857
• Organism: Bacillus cereus strain MRY14-0045

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L6485_RS12415
• Protein ID: WP_062923173.1
• Coordinates: 2424735..2425472 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L6485_RS12420
• Protein ID: WP_062923165.1
• Coordinates: 2425585..2425710 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6485_RS12390 (BCM0045_2394)	2420229..2420930	+	702	WP_236788434.1	hypothetical protein	-
L6485_RS12395 (BCM0045_2395)	2421058..2421942	+	885	WP_062923162.1	hypothetical protein	-
L6485_RS12400	2422251..2423053	-	803	Protein_2368	thiamine pyrophosphate-binding protein	-
L6485_RS12405 (BCM0045_2398)	2423160..2423642	+	483	WP_062923163.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6485_RS12410 (BCM0045_2399)	2423809..2424546	+	738	WP_062923164.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6485_RS12415 (BCM0045_2400)	2424735..2425472	+	738	WP_062923173.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6485_RS12420 (BCM0045_2401)	2425585..2425710	+	126	WP_062923165.1	hypothetical protein	Antitoxin
L6485_RS12425 (BCM0045_2402)	2425786..2425962	+	177	WP_062923166.1	stage II sporulation protein SB	-
L6485_RS12430 (BCM0045_2403)	2425981..2426370	-	390	WP_062923167.1	YxeA family protein	-
L6485_RS12435 (BCM0045_2404)	2426587..2428056	+	1470	WP_062923168.1	beta-Ala-His dipeptidase	-
L6485_RS12440 (BCM0045_2405)	2428638..2429108	-	471	WP_061530815.1	DUF5065 family protein	-
L6485_RS12445 (BCM0045_2406)	2429562..2430221	+	660	WP_000876955.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.03 Da
• Isoelectric Point: 8.3034

>T36597 WP_062923173.1 NZ_AP022857:2424735-2425472 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYIKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKTVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T36597 NZ_AP022857:2424735-2425472 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGTTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAGTTGTACGAAGAGAAGAAGCAACGGATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATATAAAAAGCTTAAACGCATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGCTCACAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCGGCAAAAACTGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAATCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT36597 WP_062923165.1 NZ_AP022857:2425585-2425710 [Bacillus cereus]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT36597 NZ_AP022857:2425585-2425710 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGATGAATTAAAAAGAAAATGA