Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 253782..254424 Replicon chromosome
Accession NZ_AP022857
Organism Bacillus cereus strain MRY14-0045

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6485_RS01435 Protein ID WP_000635963.1
Coordinates 254074..254424 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6485_RS01430 Protein ID WP_000004570.1
Coordinates 253782..254069 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6485_RS01405 (BCM0045_0230) 249098..250060 + 963 WP_236787436.1 UV DNA damage repair endonuclease UvsE -
L6485_RS01410 (BCM0045_0231) 250053..250625 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
L6485_RS01415 (BCM0045_0232) 250718..251077 + 360 WP_000635040.1 holo-ACP synthase -
L6485_RS01420 (BCM0045_0233) 251234..252184 + 951 WP_236788600.1 outer membrane lipoprotein carrier protein LolA -
L6485_RS01425 (BCM0045_0234) 252303..253472 + 1170 WP_000390600.1 alanine racemase -
L6485_RS01430 (BCM0045_0236) 253782..254069 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6485_RS01435 (BCM0045_0237) 254074..254424 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6485_RS01440 (BCM0045_0238) 254492..256660 + 2169 WP_236787437.1 Tex family protein -
L6485_RS01445 256718..256834 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6485_RS01450 (BCM0045_0239) 257030..257488 + 459 WP_062923611.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T36596 WP_000635963.1 NZ_AP022857:254074-254424 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T36596 NZ_AP022857:254074-254424 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT36596 WP_000004570.1 NZ_AP022857:253782-254069 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT36596 NZ_AP022857:253782-254069 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References